In vitro assay to demonstrate high-level erythromycin resistance of a clinical isolate of Treponema pallidum.
In vitro assay to demonstrate high-level erythromycin resistance of a clinical isolate of Treponema pallidum.
复制标题
体外测定证明梅毒螺旋体临床分离株具有高水平的红霉素耐药性。
DOI：
10.1128/aac.32.2.164
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发表时间：
1988
影响因子：
4.9
通讯作者：
BassfordJr,PJ
中科院分区：
文献类型：
--
作者：
Stamm,LV;Stapleton,JT;BassfordJr,PJ
我们以前已经证明，从感染的兔睾丸中新鲜提取的梅毒螺旋体细胞可以用[35 S]甲硫氨酸进行固有放射性标记，达到非常高的比活度。在这项研究中，我们使用的[35 S]蛋氨酸掺入三氯乙酸沉淀蛋白在体外的抑制作为一种测定，以测试三种不同的致病性密螺旋体菌株对各种抗生素的敏感性。总的来说，这些结果与这些抗生素治疗人类梅毒患者的已知疗效非常相关。然而，其中一种测试菌株是临床分离的T。苍白球命名为街菌株14，被发现表现出对红霉素和密切相关的大环内酯类药物罗红霉素（RU 965）的高水平抗性。Street菌株14最初分离自一名对红霉素治疗无效的活动性二期梅毒患者。因此，我们的结果表明T.苍白球可导致红霉素治疗失败。此外，通过该测定发现街道菌株14密螺旋体对多种抗生素的敏感性通常低于T. pallidum Nichols菌株这些发现表明，街道菌株14密螺旋体的外被膜对抗生素的渗透性通常低于Nichols菌株密螺旋体。
We have previously demonstrated that cells of Treponema pallidum freshly extracted from infected rabbit testes can be intrinsically radiolabeled with [35 S]methionine to very high specific activities. In this study we used the inhibition of [35 S]methionine incorporation into trichloroacetic acid-precipitable protein in vitro as an assay to test the susceptibilities of three different pathogenic treponemal strains to various antibiotics. In general, the results correlated very well with the known efficacies of these antibiotics in treating human patients with syphilis. One of the strains tested, however, a clinical isolate of T. pallidum designated street strain 14, was found to exhibit high-level resistance to erythromycin and a closely related macrolide, roxithromycin (RU 965). Street strain 14 was originally isolated from a human patient with active secondary syphilis who failed to respond to erythromycin therapy. Thus, our results indicate that an erythromycin-resistant strain of T. pallidum can be responsible for erythromycin treatment failure. In addition, street strain 14 treponemes were found to be generally less susceptible by this assay to a variety of antibiotics than were treponemes of the T. pallidum Nichols strain. These findings suggest that the outer envelope of street strain 14 treponemes may be generally less permeable to antibiotics than is that of Nichols strain treponemes.