Uncaging calcium in neurons.
Uncaging calcium in neurons.
复制标题
释放神经元中的钙。
DOI：
10.1101/pdb.top079491
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发表时间：
2013
影响因子：
--
通讯作者：
V. Shahrezaei
中科院分区：
文献类型：
--
作者：
K. Delaney;V. Shahrezaei
Changes in intracellular free calcium concentration (Δ[Ca(2+)]i) driving physiological events such as neurotransmitter release or Ca(2+)-dependent currents can be monitored using Ca(2+)-sensitive fluorescent dyes. Although these dyes can correlate Δ[Ca(2+)]i with a physiological event, they cannot directly test for causality between changes in [Ca(2+)]i and that event. Photolabile Ca(2+) chelators are Ca(2+)-binding molecules that can alter and, to a certain extent, control [Ca(2+)]i in an inducible manner and with temporal and spatial resolution that surpasses microinjection or ionophore application. Here we discuss the properties of caged Ca(2+) compounds as well as some practical considerations for their use in neuronal cells, where they have proven particularly effective.
七鳃鳗网状脊髓轴突的急性分离，以便能够从各个功能性突触前末梢的释放面膜进行记录。
DOI：
10.3791/51925
发表时间：
2014
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Ramachandran,Shankar;Alford,Simon
通讯作者：
Alford,Simon