Evaluation of methoprene effect on Aedes aegypti (Diptera: Culicidae) development in laboratory conditions
Evaluation of methoprene effect on Aedes aegypti (Diptera: Culicidae) development in laboratory conditions
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DOI：
10.1590/s0074-02762005000400016
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发表时间：
2005-07-01
期刊：
影响因子：
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通讯作者：
Valle, Denise
中科院分区：
文献类型：
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作者：
Braga, Ima Aparecida;Mello, Cícero Brasileiro;Valle, Denise
巴西的几个埃及伊蚊种群对幼虫科的temephos有抗性。抑制成虫羽化的甲氧普烯是巴西登革热控制方案设想的替代品之一。然而，在巴西，病媒感染率是通过幼虫指数来衡量的，有人声称，在实地使用烯虫酯可能会面临操作问题。为了确定一个标准化的协议，甲氧普烯的效果进行了评价，在实验室条件下连续暴露的幼虫（洛克菲勒株）的甲氧普烯配方提供给PNCD。甲氧普烯衍生的死亡率主要发生在蛹期，蛹的发育与甲氧普烯浓度成反比。给药组和对照组雌性动物产卵的数量和活力相当。甲氧普烯剂量依赖性延迟的发展，但强烈的相关性，发现总死亡率或成人出现抑制，如果获得的数据时，所有控制蚊子出现相比，获得的数据时，甲氧普烯处理组完成发展。在控制成虫出现时，总的甲氧普仑诱导的死亡率的累积记录，建议用于现场种群的常规评价。死亡率的所有标本，但不是幼虫，可以解释成人出现抑制，确认幼虫指标的不足，以评估甲氧普烯的效果。
Several Brazilian Aedes aegypti populations are resistant to the larvicidae temephos. Methoprene, that inhibits adult emergence, is one of the alternatives envisaged by the Brazilian Dengue Control Program (PNCD). However at Brazil vector infestation rates are measured through larvae indexes and it has been claimed that methoprene use in the field could face operational problems. In order to define a standardized protocol, methoprene effect was evaluated in laboratory conditions after continuous exposure of larvae (Rockefeller strain) to a methoprene-formulation available to the PNCD. Methoprene-derived mortality occurs mainly at the pupa stage and pupa development is inversely proportional to methoprene concentration. Number and viability of eggs laid by treated and control females are equivalent. A methoprene dose-dependent delay in the development was noted; however strong correlations were found for total mortality or adult emergence inhibition if data obtained when all control mosquitoes have emerged are compared to data obtained when methoprene-treated groups finish development. The cumulative record of total methoprene-induced mortality at the time control adults emerge is proposed for routine evaluation of field populations. Mortality of all specimens, but not of larva, could account for adult emergence inhibition, confirming the inadequacy of larvae indexes to evaluate methoprene effect.