5-nitro-gamma-tocopherol increases in human plasma exposed to cigarette smoke in vitro and in vivo.
5-nitro-gamma-tocopherol increases in human plasma exposed to cigarette smoke in vitro and in vivo.
复制标题
体外和体内暴露于香烟烟雾的人血浆中的 5-硝基-γ-生育酚都会增加。
DOI：
10.1016/j.freeradbiomed.2003.09.010
复制
发表时间：
2003
影响因子：
7.4
通讯作者：
Traber,MaretG
中科院分区：
文献类型：
--
作者：
Leonard,ScottW;Bruno,RichardS;Paterson,Elaine;Schock,BettinaC;Atkinson,Jeffery;Bray,TammyM;Cross,CarrollE;Traber,MaretG
We hypothesized that the high concentrations of reactive nitrogen species in cigarette smoke and the known stimulatory effects of cigarette smoke on the inflammatory immune systems would lead to the formation of 5-nitro-γ-tocopherol (NGT). In order to assess γ-tocopherol nitration, human plasma was exposed in vitro to gas phase cigarette smoke (GPCS) or air for up to 6 h. A liquid chromatography-mass spectrometry (LC-MS) method was developed to quantitate NGT. Detector response was linear from 0.1 to 3 pmol NGT, with a detection limit of 20 fmol. After a 1 h lag time, 6 h plasma exposure to GPCS depleted ∼ 75% of α-T, ∼ 60% of γ-T and increased NGT from 3 to 134 nmol/l. The increase in NGT accounted for ∼ 20% of the γ-T decrease. NGT also correlated (R2= 0.9043) with nitrate concentrations in GPCS-exposed plasma. The physiologic relevance of NGT was evaluated in a group of healthy humans. Smokers (n = 15) had plasma NGT concentrations double those of nonsmokers (n = 19), regardless of corrections using lipids or γ-T; plasma α-T and γ-T concentrations were similar between the groups. Our results show that LC-MS can be successfully used for NGT quantitation in biologic samples. Importantly, NGT in smokers' plasma suggests that cigarette smoking causes increased nitrosative stress.
