Microbacterium gubbeenense sp nov., from the surface of a smear-ripened cheese
Microbacterium gubbeenense sp nov., from the surface of a smear-ripened cheese
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DOI：
10.1099/00207713-51-6-1969
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发表时间：
2001-11-01
影响因子：
2.8
通讯作者：
Fox, PF
中科院分区：
文献类型：
--
作者：
Brennan, NM;Brown, R;Fox, PF
对从涂抹成熟的奶酪表面分离的11株微生物样生物进行了表型和系统发育研究。菌株为革兰氏阳性、过氧化氢酶阳性、兼性厌氧、氧化酶阴性、不产芽孢、不运动、小而细长的杆状菌，在12% (w/v) NaCl条件下生长。化学分类调查表明，所有分离物均属于微细菌属。它们含有以l -赖氨酸为二氨基酸型和乙二醇酰酰基型的B1型肽聚糖；鼠李糖和半乳糖是细胞壁糖。G+C含量在69 ~ 72 mol%之间。甲基萘醌类化合物主要为MK-11和MK-12，脂肪酸主要为iso C15:0、C17:0和iso C16:0。4个分离株的16S rRNA序列系统发育分析表明，它们代表了微杆菌属的一个新的亚系，巴氏微杆菌是它们最近的系统发育邻居。巴氏分枝杆菌与分离株序列相似性最高；然而，DNA-DNA杂交显示分离株与巴氏分枝杆菌的染色体相似性仅为38%。根据分离菌株的系统发育和表型特征，建议将其分类为一个新的微细菌种，并将其命名为Microbacterium gubbenense sp. 11。沉积型菌株为LMG S-19263(T) （= NCIMB 30129(T)）。该型菌株16S rDNA序列的GenBank登录号为AF263563。
Phenotypic and phylogenetic studies were performed on 11 strains of a Microbacterium-like organism isolated from the surface of a smear-ripened cheese. The isolates were Gram-positive, catalase-positive, facultatively anaerobic, oxidase-negative, non-spore-forming, non-motile, small, slender rods and grew in 12% (w/v) NaCl. Chemotaxonomic investigation revealed that all the isolates belonged unambiguously to the genus Microbacterium. They contained type B1 peptidoglycans with L-lysine as the diamino acid and glycolyl acyl types; rhamnose and galactose were the cell wall sugars. The G+C content ranged from 69 to 72 mol%. The major menaquinones were MK-11 and MK-12 and the major fatty acids were anteiso C15:0 and C17:0 and iso C16:0. Phylogenetic analysis of the 16S rRNA sequences of four isolates showed that they represented a new subline in the genus Microbacterium, with Microbacterium barkeri as their nearest phylogenetic neighbour. M. barkeri showed the highest sequence similarity to the isolates; however, DNA-DNA hybridization showed that the isolates had only 38% chromosomal similarity to M. barkeri. Based on the phylogenetic and phenotypic distinctiveness of the isolates, it is proposed that they be classified as a new Microbacterium species, for which the name Microbacterium gubbeenense sp. nov. is suggested. The type strain has been deposited as LMG S-19263(T) (= NCIMB 30129(T)). The GenBank accession number for the 16S rDNA sequence of the type strain is AF263563.