Metabolic profiling of a Rhizopus oryzae fumaric acid production mutant generated by femtosecond laser irradiation
Metabolic profiling of a Rhizopus oryzae fumaric acid production mutant generated by femtosecond laser irradiation
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飞秒激光照射产生的米根霉富马酸生产突变体的代谢分析
DOI：
10.1016/j.biortech.2012.03.087
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发表时间：
2012-06-01
影响因子：
11.4
通讯作者：
Jia, Xiaoqiang
中科院分区：
文献类型：
--
作者：
Yu, Shouzhi;Huang, Di;Jia, Xiaoqiang
利用飞秒激光辐照诱导根霉突变，提高富马酸产量。与亲本菌株相比，突变株FM 19的效价和产量分别提高了56.3%和36.6%，对应于49.4g/L的效价和0.56g富马酸/g葡萄糖的产量。气相色谱-质谱联用分析表明，高产菌株的碳代谢（Embden-Meyerhof-Parnas循环和三羧酸循环）和氨基酸代谢水平较高，特别是4-氨基丁酸和5-氨基乙酰丙酸在稳定期分别比亲本菌株提高了10.33倍和7.22倍。这些发现为高产富马酸R的代谢特征提供了新的见解。米。(C)2012爱思唯尔有限公司保留所有权利。
Femtosecond laser irradiation was employed to induce mutations in Rhizopus oryzae, leading to increases in fumaric acid production. Compared to the parental strain, mutant strain FM19 exhibited an increase in titer and yield of 56.3% and 36.6%, respectively, corresponding to a titer of 49.4 g/L and a yield of 0.56 g fumaric acid per g glucose. Metabolic profiling by gas chromatography-mass spectrometry revealed that higher levels of carbon (Embden-Meyerhof-Parnas and tricarboxylic acid cycle) and amino acid metabolism were operating in the high-yielding strain: particularly, 4-aminobutyric acid and 5-aminolevulinic acid were increased 10.33- and 7.22-fold, respectively, compared with parental strain during stationary phase. These findings provided new insights into metabolic characterization of high-yielding fumaric acid R. oryzae. (C) 2012 Elsevier Ltd. All rights reserved.