A comparison of mechanical properties derived from multiple skeletal sites in mice
A comparison of mechanical properties derived from multiple skeletal sites in mice
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DOI：
10.1016/j.jbiomech.2004.04.020
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发表时间：
2005-03-01
影响因子：
2.4
通讯作者：
Turner, CH
中科院分区：
文献类型：
--
作者：
Schriefer, JL;Robling, AG;Turner, CH
实验小鼠为骨骼生物力学研究提供了一种通用的实验模型。为了确定小鼠骨骼的强度，已经使用几种程序对各种骨骼进行了机械测试。由于测试方法的差异，以前研究的数据不具有可比性。本研究的目的是确定哪种长骨提供的值最接近已发表的骨材料特性，同时还提供可靠和可重现的结果。为此，在三点弯曲下对低骨量C57 BL/6 H（B6）和高骨量C3 H/HeJ（C3 H）小鼠的股骨、肱骨、第三跖骨、桡骨和胫骨进行机械测试。生物力学测试结果显示，不同骨种和不同品系小鼠的测试结果存在显著性差异（P <0. 05）。建立了股骨、跖骨和桡骨的计算模型，以直观地显示三点弯曲测试中固有的测量误差类型。模型表明，加载点局部变形、剪切变形和圆柱截面环形变形会引起测量误差。增加纵横比（骨长度/宽度）改善了两种小鼠品系的骨的杨氏模量的测量（p < 0.01）。具有最高纵横比和最大皮质厚度与半径比的骨更适合弯曲测试，因为在计算模型中观察到的测量误差较小。在测试的骨骼中，半径是机械测试的首选，因为它具有高纵横比、最小测量误差和低变异性。(C)2004 Elsevier Ltd.保留所有权利。
Laboratory mice provide a versatile experimental model for studies of skeletal biomechanics. In order to determine the strength of the mouse skeleton, mechanical testing has been performed on a variety of bones using several procedures. Because of differences in testing methods, the data from previous studies are not comparable. The purpose of this study was to determine which long bone provides the values closest to the published material properties of bone, while also providing reliable and reproducible results. To do this, the femur, humerus, third metatarsal, radius, and tibia of both the low bone mass C57BL/6H (B6) and high bone mass C3H/HeJ (C3H) mice were mechanically tested under three-point bending. The biomechanical tests showed significant differences between the bones and between mouse strains for the five bones tested (P < 0.05).Computational models of the femur, metatarsal, and radius were developed to visualize the types of measurement error inherent in the three-point bending tests. The models demonstrated that measurement error arose from local deformation at the loading point, shear deformation and ring-type deformation of the cylindrical cross-section. Increasing the aspect ratio (bone length/width) improved the measurement of Young's modulus of the bone for both mouse strains (p < 0.01). Bones with the highest aspect ratio and largest cortical thickness to radius ratio were better for bending tests since less measurement error was observed in the computational models. Of the bones tested, the radius was preferred for mechanical testing because of its high aspect ratio, minimal measurement error, and low variability. (C) 2004 Elsevier Ltd. All rights reserved.