Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice
Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice
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DOI：
10.1016/j.vaccine.2005.06.016
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发表时间：
2005-10-25
期刊：
影响因子：
5.5
通讯作者：
Fang, WH
中科院分区：
文献类型：
--
作者：
Yang, ZG;Sun, HX;Fang, WH
本研究探讨了黄芪皂苷（AMS）的溶血活性及其对免疫小鼠抗卵清蛋白（OVA）的细胞免疫和体液免疫的增强作用。用0.5%兔红细胞测定AMS的溶血活性。AMS有轻微的溶血作用，浓度为500 μ g/ml时溶血率为0.66%。此外，还研究了三个剂量的AMS对ICR小鼠抗卵清蛋白（OVA）的细胞免疫和体液免疫的佐剂效应。在第1天和第15天，用单独的OVA 100 μ g或用溶解在含有明矾（200 μ g）、基拉（10和20 μ g）或AMS（50、100或200 μ g）的盐水中的OVA 100 μ g皮下免疫ICR小鼠。两周后（第28天），测定伴刀豆球蛋白A（Con A）、脂多糖（LPS）和OVA刺激的脾细胞增殖和血清中的OVA特异性抗体。AMS能显著促进ConA、LPS和OVA诱导的小鼠脾细胞增殖，尤以100 μ g剂量组为显著（P < 0.05或P < 0.001）。与对照组相比，AMS组血清中OVA特异性IgG、IgG 1和IgG 2b抗体滴度也显著升高（P < 0.01或P < 0.001）。此外，AMS和基拉对小鼠体内OVA特异性IgG、IgG 1和IgG 2b抗体应答的增强作用无显著差异（P > 0.05）。结果提示，AMS可作为低溶血性或无溶血性的免疫佐剂。(c)2005爱思唯尔有限公司保留所有权利。
In this study, the haemolytic activities of Astragalus membranaceus saponins (AMS) and its adjuvant potentials on the cellular and humoral immune responses of ICR mice against OVA were evaluated. We determined the haemolytic activity of AMS using 0.5% rabbit red blood cell. AMS showed a slight haemolytic effect, with its haemolytic percents being 0.66% at the concentration of 500 mu g/ml. Furthermore, the adjuvant potentials of AMS at three dose levels on the cellular and humoral immune responses of ICR mice against ovalbumin (OVA) were investigated. ICR mice were immunized subcutaneously with OVA 100 mu g alone or with OVA 100 mu g dissolved in saline containing Alum (200 mu g), QuilA (10 and 20 mu g) or AMS (50, 100 or 200 mu g) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. AMS significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice especially at a dose of 100 mu g (P < 0.05 or P < 0.001). OVA-specific IgG, IgG1 and IgG2b antibody titers in serum were also significantly enhanced by AMS compared with OVA control group (P < 0.01 or P < 0.001). Moreover, no significant differences (P > 0.05) were observed between enhancing effect of AMS and QuilA on the OVA-specific IgG, IgG1 and IgG2b antibody responses to OVA in mice. In conclusion, the results suggest that AMS could be safely used as adjuvant with low or non-haemolytic effect. (c) 2005 Elsevier Ltd. All rights reserved.