The 2'-O-methyladenosine nucleoside modification gene OsTRM13 positively regulates salt stress tolerance in rice.
The 2'-O-methyladenosine nucleoside modification gene OsTRM13 positively regulates salt stress tolerance in rice.
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DOI：
10.1093/jxb/erx061
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发表时间：
2017-03-01
影响因子：
6.9
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Wang Y;Li D;Gao J;Li X;Zhang R;Jin X;Hu Z;Zheng B;Persson S;Chen P
OsTRM13是一个2′-O-甲基腺苷tRNA核苷修饰基因，可正向调节水稻的耐盐胁迫能力。压力会引起 tRNA 中修饰核苷的变化，这些变化会影响密码子-反密码子相互作用，从而影响目标蛋白的翻译。某些核苷修饰基因与植物的胁迫耐受性和免疫反应的调节有关。在这项研究中，我们发现在盐胁迫和脱落酸（ABA）处理下的水稻幼苗中，2'-O-甲基腺苷（Am）核苷显着增加。我们确定 LOC_Os03g61750 (OsTRM13) 作为 Am 修饰的水稻候选甲基转移酶。 OsTRM13 转录水平在盐胁迫和 ABA 处理后显着增加，并且发现 OsTrm13 蛋白主要位于细胞核。更重要的是，OsTRM13过表达植物表现出提高的盐胁迫耐受性，反之亦然，OsTRM13 RNA干扰（RNAi）植物表现出耐受性降低。此外，OsTRM13 补充了 Am 合成缺陷的酵母 trm13Δ 突变体，并且纯化的 OsTrm13 蛋白在体外催化 tRNA-Gly-GCC 上 Am 核苷的形成。我们的研究结果表明，编码水稻 tRNA 核苷甲基转移酶的 OsTRM13 是水稻耐盐胁迫的重要调节因子。
OsTRM13 is a 2′-O-methyladenosine tRNA nucleoside modification gene that positively regulates salt stress tolerance in rice. Stress induces changes of modified nucleosides in tRNA, and these changes can influence codon–anticodon interaction and therefore the translation of target proteins. Certain nucleoside modification genes are associated with regulation of stress tolerance and immune response in plants. In this study, we found a dramatic increase of 2′-O-methyladenosine (Am) nucleoside in rice seedlings subjected to salt stress and abscisic acid (ABA) treatment. We identified LOC_Os03g61750 (OsTRM13) as a rice candidate methyltransferase for the Am modification. OsTRM13 transcript levels increased significantly upon salt stress and ABA treatment, and the OsTrm13 protein was found to be located primarily to the nucleus. More importantly, OsTRM13 overexpression plants displayed improved salt stress tolerance, and vice versa, OsTRM13 RNA interference (RNAi) plants showed reduced tolerance. Furthermore, OsTRM13 complemented a yeast trm13Δ mutant, deficient in Am synthesis, and the purified OsTrm13 protein catalysed Am nucleoside formation on tRNA-Gly-GCC in vitro. Our results show that OsTRM13, encoding a rice tRNA nucleoside methyltransferase, is an important regulator of salt stress tolerance in rice.