Mitochondrial DNA content and 4977 bp deletion in unfertilized oocytes
Mitochondrial DNA content and 4977 bp deletion in unfertilized oocytes
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DOI：
10.1093/molehr/gah243
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发表时间：
2005-12-01
影响因子：
4
通讯作者：
Ho, PC
中科院分区：
文献类型：
--
作者：
Chan, CCW;Liu, VWS;Ho, PC
以前的研究分析了未受精卵母细胞中线粒体DNA（mtDNA）缺失的发生率和mtDNA含量与供体年龄的关系，但一直存在争议。本研究的目的是比较未受精卵母细胞的这两个参数，并将它们与供体的年龄联系起来。52名妇女捐赠了155个未受精的中期II（MII）卵母细胞。4977 bp缺失发生率为34.6%，mtDNA拷贝数为598350 +/-265862。与年轻女性相比，≥ 35岁女性4977 bp缺失的发生率显著较高，mtDNA拷贝数较低，FSH水平较高，卵巢反应较差。mtDNA拷贝数与供体年龄呈负相关。老年妇女线粒体DNA缺失发生率高，拷贝数低，提示这两个参数可能反映卵巢老化。
Previous studies analysing the incidences of mitochondrial DNA (mtDNA) deletions and mtDNA content in unfertilized oocytes in relation to donors' age have been controversial. The objective of the study was to compare these two parameters in unfertilized oocytes and relate them to the donors' age. Fifty-two women donated 155 unfertilized metaphase II (MII) oocytes. The incidence of 4977 bp deletion was 34.6%, and the mtDNA copy number was 598 350 +/- 265 862. Women >= 35 years of age had a significantly higher incidence of 4977 bp deletion, lower mtDNA copy number, higher FSH level and poorer ovarian response when compared with younger women. The mtDNA copy number was negatively correlated with the donor's age. The higher incidence of mtDNA deletion and lower mtDNA copy number in older women suggested that these two parameters may reflect ovarian ageing.