The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells
The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells
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DOI：
10.1016/j.virol.2008.05.036
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发表时间：
2008-09-15
期刊：
影响因子：
3.7
通讯作者：
Burzio, Luis O.
中科院分区：
文献类型：
--
作者：
Goic, Bertsy;Bustamante, Juanita;Burzio, Luis O.
传染性鲑鱼贫血病毒（Infectious salmon anemia virus，ISAV）属于正粘病毒科（Orthomyxoviridae）的一个新属，是鲑鱼养殖业的重要病原。用核蛋白（NP）特异性单克隆抗体进行的间接免疫荧光测定显示感染细胞中亚细胞区室的差异染色。特别令人感兴趣的是核仁的染色，其在感染ISAV的CHSE-214、EPC和SHK-1细胞中显示与核仁素共定位。这些结果证实了共免疫沉淀研究显示NP和nucleclin之间的相互作用。此外，用ISAV的8个RNA片段中的每一个的特异性探针进行的原位杂交表明，基因组链和反基因组链也定位于核仁中。这些结果表明核仁在ISAV基因组的复制和/或包装中的作用。(C)2008年爱思唯尔公司All rights reserved.
The infectious salmon anemia Virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleclin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results Suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome. (C) 2008 Elsevier Inc. All rights reserved.