Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis.

Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis.
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枯草芽孢杆菌中 sigma H RNA 聚合酶使用的启动子的克隆。

DOI:
10.1016/0378-1119(90)90347-t
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发表时间:
1990
期刊:
影响因子:
3.5
通讯作者:
MoranJr,CP
MoranJr,CP
中科院分区:
生物学3区
文献类型:
--
作者:
Carter3rd,HL;Tatti,KM;MoranJr,CP

文献摘要

被引文献

相似文献

次级RNA聚合酶σ因子σ H是枯草芽孢杆菌内生孢子发育所必需的。然而,只有少数启动子被RNA聚合酶使用含有σ H(E σ H)已被确定。我们使用体外转录的随机克隆片段的B。枯草芽孢杆菌染色体DNA,以鉴定由E σ H使用的启动子。该启动子在孢子形成开始之前是有活性的。
The secondary RNA polymerase sigma factor σ H is essential for endospore development in Bacillus subtilis. However, only a few promoters that are used by RNA polymerase containing σ H (E σ H) have been identified. We used in vitro transcription of random cloned fragments of B. subtilis chromosomal DNA to identify a promoter that is used by E σ H. This promoter is active before the onset of sporulation.