Development of poly(anhydride) nanoparticles loaded with peanut proteins: The influence of preparation method on the immunogenic properties
Development of poly(anhydride) nanoparticles loaded with peanut proteins: The influence of preparation method on the immunogenic properties
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DOI：
10.1016/j.ejpb.2012.06.014
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发表时间：
2012-10-01
影响因子：
4.9
通讯作者：
Gamazo, Carlos
中科院分区：
文献类型：
--
作者：
De Souza Reboucas, Juliana;Manuel Irache, Juan;Gamazo, Carlos
过敏原特异性免疫疗法基于过敏原的施用，其主要缺点是诱导过敏反应。在此背景下，我们报告了一种佐剂和过敏原传递系统的产生与减少IgE诱导花生过敏原免疫治疗。因此，我们使用溶剂置换法制备并表征了负载有花生蛋白的聚（酸酐）纳米颗粒，并在制造过程中进行了一些修改。聚合物的沉淀用乙醇和水的混合物或水进行。分别通过冷冻干燥或喷雾干燥来干燥所得纳米颗粒。成功开发了负载花生蛋白的聚酸酐纳米颗粒，实现了高包封率（70-80%）和制造产率（60-80%）。用聚酸酐纳米粒包裹花生蛋白皮内免疫小鼠（C57 B1/6）后，观察到强烈的T（H）1/T（H）2混合型免疫应答。此外，据我们所知，我们还首次提供了明确的证据，证明了配方设计对纳米颗粒免疫刺激特性的影响。总而言之，我们的研究结果表明，聚（酸酐）纳米颗粒是免疫反应的有效刺激剂，并且是应用于免疫治疗的有前途的佐剂和过敏原递送系统。(C)2012 Elsevier B. V.保留所有权利。
Allergen-specific immunotherapy is based on the administration of allergens with the main disadvantage of inducing an allergic reaction. Within this context, we report the generation of an adjuvant and allergen-delivery system for peanut allergen immunotherapy with reduced IgE induction. Therefore, we prepared and characterized poly(anhydride) nanoparticles loaded with peanut proteins using the solvent displacement method, with some modifications in the manufacturing process. The precipitation of polymer was performed with either a mixture of ethanol and water or water. The resultant nanoparticles were dried by either freeze-drying or spray-drying, respectively. Poly(anhydride) nanoparticles loaded with peanut proteins were successfully developed, achieving both high encapsulation efficiency (70-80%) and manufacturing yield (60-80%). After intradermal immunization of mice (C57Bl/6) with peanut proteins incorporated into poly(anhydride) nanoparticles, a strong mixed T(H)1/T(H)2-type immune response was observed. Furthermore, we also provide, to our knowledge for the first time, clear evidence of the influence of formulation design on the immunostimulatory properties of nanoparticles. Taken together, our findings indicate that poly(anhydride) nanoparticles are efficient stimulators of immune responses and promising adjuvants and allergen-delivery systems applied for immunotherapy. (C) 2012 Elsevier B.V. All rights reserved.