Sooty mangabey (Cercocebus torquatus atys) IGHG and IGHA genes.
Sooty mangabey (Cercocebus torquatus atys) IGHG and IGHA genes.
复制标题
乌白眉猴 (Cercocebus torquatus atys) IGHG 和 IGHA 基因。
DOI：
10.1007/s00251-006-0152-z
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发表时间：
2006
期刊：
影响因子：
3.2
通讯作者：
Attanasio,Roberta
中科院分区：
文献类型：
--
作者：
Scinicariello,Franco;Masseoud,Feda;Jayashankar,Lakshmi;Attanasio,Roberta
Antibodies are adaptor molecules of the immune system that link antigen recognition with the effector mechanisms responsible for antigen clearance. Several nonhuman primate species are widely used in biomedical research, especially for vaccine development and for AIDS-related studies. However, nonhuman primate antibody molecules have been characterized only partially and only in a few species. Here, we describe sooty mangabey (Cercocebus torquatus atys)IGHGandIGHAgenes, which encode the heavy-chain constant region of IgG and IgA molecules, respectively. The four mangabeyIGHGgenes are highly homologous to the rhesus macaque and baboonIGHGgenes (percent identity varies between 94.0 and 98.8, depending on the subclass), with most amino acid differences located in the hinge regions. Results obtained by real-time reverse transcription polymerase chain reaction show that the fourIGHGgenes are expressed at least at the mRNA level. The mangabeyIGHAgene is highly homologous to the corresponding gene from rhesus macaques (percent identity ranges from 88.6 to 96.7, depending on the allele considered), the only other nonhominoid primate species for which the complete sequence of theIGHAgene is currently available. In the mangabey analyzed, twoIGHAalleles are present, confirming that high levels ofIGHAgene heterozygosity are present in monkey species. These results show that nonhuman primate gamma and alpha heavy chains differ from each other mostly at the level of the hinge region and that alpha sequence heterogeneity in nonhuman primate species is also present in other gamma regions. In addition, these results provide sequence information that can be used for residue frequency analysis of antibody heavy-chain constant region sequences.
