Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin
Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin
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DOI：
10.1016/j.joen.2008.04.006
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发表时间：
2008-07-01
影响因子：
4.2
通讯作者：
Lim, Chwee-Teck
中科院分区：
文献类型：
--
作者：
Kishen, Anil;Sum, Chee-Peng;Lim, Chwee-Teck
粪肠球菌常与治疗后牙髓感染有关。因为细菌粘附到基质上是生物膜形成的最早阶段，所以引出将这种细菌粘附到牙本质上的因素将有助于理解其与治疗失败的根管的关联。本研究旨在探讨根管冲洗液对E.粪便到牙本质。采用荧光显微镜进行细菌粘附试验，采用原子力显微镜测量粘附力。乙二胺四乙酸（EDTA）冲洗牙本质后，牙本质的粘附性和粘附力显著增加，次氯酸钠（NaOCl）冲洗牙本质后，牙本质的粘附力显著降低，而洗必泰（CHX）冲洗牙本质后，牙本质的粘附力增加，但粘附细菌数量减少。EDTA、NaOCl和CHX的冲洗方案导致粘附的E.粪细胞本研究强调了改变牙本质物理化学性质的化学物质将影响E.粪便到牙本质。
Enterococcus faecalis is frequently associated with post-treatment endodontic infections. Because adherence of bacteria to a substrate is the earliest stage in biofilm formation, eliciting the factors that links adherence of this bacterium to dentin would help in understanding its association with treatment-failed root canals. This investigation aimed to study the effects of endodontic irrigants on the adherence of E. faecalis to dentin. The bacteria adherence assay was conducted by using fluorescence microscopy, and the adhesion force was measured by using atomic force microscopy. There were significant increases in adherence and adhesion force after irrigation of dentin with ethylenediaminetetraacetic acid (EDTA), whereas sodium hypochlorite (NaOCl) reduced it. With the use of chlorhexidine (CHX), the force of adhesion increased, but the adherence assay showed a reduction in the number of adhering bacteria. The irrigation regimen of EDTA, NaOCl, and CHX resulted in the least number of adhering E. faecalis cells. This study highlighted that chemicals that alter the physicochemical properties of dentin will influence the nature of adherence, adhesion force, and subsequent biofilm formation of E. faecalis to dentin.