L-arginine and free radical scavengers increase cerebral blood flow and brain tissue nitric oxide concentrations after controlled cortical impact injury in rats
L-arginine and free radical scavengers increase cerebral blood flow and brain tissue nitric oxide concentrations after controlled cortical impact injury in rats
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DOI：
10.1089/08977150360517209
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发表时间：
2003-01-01
影响因子：
4.2
通讯作者：
Robertson, CS
中科院分区：
文献类型：
--
作者：
Cherian, L;Robertson, CS
为探讨脑外伤后给予L-精氨酸引起脑血流量增加的机制，在受控皮质冲击损伤模型中比较L-精氨酸、D-精氨酸以及自由基清除剂超氧化物歧化酶(SOD)和过氧化氢酶对脑血流动力学的影响。用异氟烷麻醉动物。测量的参数包括平均血压、颅内压、使用激光多普勒血流计 (LDF) 的脑血流量以及使用 NO 电极的脑组织一氧化氮 (NO) 浓度。与给予盐水的对照动物相比，施用 L-精氨酸（而非 D-精氨酸）导致影响部位的组织 NO 浓度显着增加，并且 LDF 改善。单独使用 SOD 以及与过氧化氢酶联合使用会导致脑组织 NO 浓度显着增加。然而，只有同时给予 SOD 和过氧化氢酶时，LDF 才会持续改善。这些研究支持这样的理论：L-精氨酸给药通过增加一氧化氮的产生来改善创伤后脑血流量。创伤后自由基的产生也可能通过灭活 NO 来减少 CBF。
To examine the mechanism of the increase in cerebral blood flow induced by L-arginine administration after traumatic brain injury, the cerebral hemodynamic effects Of L-arginine, D-arginine, and the free radical scavengers superoxide dismutase (SOD) and catalase were compared in the controlled cortical impact injury model in rats. Animals were anesthetized with isoflurane. Measured parameters included mean blood pressure, intracranial pressure, cerebral blood flow using laser Doppler flowmetry (LDF) and brain tissue nitric oxide (NO) concentrations using an NO electrode. L-arginine, but not D-arginine, administration resulted in a significant increase in tissue NO concentrations and an improvement in LDF at the impact site, compared to control animals given saline. Administration of SOD alone and in combination with catalase resulted in a significant increase in brain tissue NO concentrations. However, LDF was consistently improved only when both SOD and catalase were given. These studies support the theory that L-arginine administration improves post-traumatic cerebral blood flow by increasing NO production. Free radical production after trauma may also contribute to the reduction in CBF by inactivating NO.