Plasma Deactivation of Endotoxic Biomolecules: Vacuum Ultraviolet Photon and Radical Beam Effects on Lipid A
Plasma Deactivation of Endotoxic Biomolecules: Vacuum Ultraviolet Photon and Radical Beam Effects on Lipid A
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内毒素生物分子的等离子体失活：真空紫外光子和自由基束对脂质 A 的影响
DOI：
10.1002/ppap.201200087
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发表时间：
2013
影响因子：
3.5
通讯作者：
G. Oehrlein
中科院分区：
文献类型：
--
作者：
T. Chung;N. Ning;J. Chu;D. Graves;E. Bartis;Joonil Seog;G. Oehrlein
人们普遍认为，等离子体产生的高能和反应性物质是等离子体诱导灭菌的原因;然而，这些物质如何单独或协同作用以灭活内毒素生物分子尚未完全了解。使用真空束系统，我们研究了真空紫外线（VUV）辐射、氧和氘自由基对脂多糖免疫刺激区脂质A的影响。VUV诱导的光分解导致暴露的脂质A膜的本体改性，直至VUV光子的穿透深度，约200 nm。虽然自由基诱导的脂质A的蚀刻产率低于VUV诱导的光解，但二次离子质谱和基于人全血的测定表明，自由基在膜表面产生更高程度的改性。本研究有助于从根本上了解等离子体对生物分子的影响，以便更好地失活方案和应用。
It is widely accepted that plasma‐generated energetic and reactive species are responsible for plasma‐induced sterilization; however, how these species act alone or synergistically to deactivate endotoxic biomolecules is not completely understood. Using a vacuum beam system, we study the effects of vacuum ultraviolet (VUV) radiation, oxygen and deuterium radicals on lipid A, the immune‐stimulating region of lipopolysaccharide. VUV‐induced photolysis causes bulk modification of exposed lipid A film up to the penetration depth of VUV photons, ≈200 nm. Although radical‐induced etch yield of lipid A is lower than VUV‐induced photolysis, secondary ion mass spectrometry and human whole blood‐based assay suggest that radicals render a higher degree of modification at the film surface. This study contributes to the fundamental understanding of plasma effects on biomolecules for a better deactivation scheme and applications.
影响因子：
3.3
作者：
Nobre, M.;Fernandes, A.;Limao-Vieira, P.
通讯作者：
Limao-Vieira, P.