Evolution and Diversity of Pre-mRNA Splicing in Highly Reduced Nucleomorph Genomes
Evolution and Diversity of Pre-mRNA Splicing in Highly Reduced Nucleomorph Genomes
复制标题
高度还原核型基因组中前体 mRNA 剪接的进化和多样性
DOI：
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发表时间：
2018
影响因子：
3.3
通讯作者：
N. M. Fast
中科院分区：
文献类型：
--
作者：
D. K. Wong;C. Grisdale;N. M. Fast
真核生物基因被内含子打断，内含子在被称为前mrna剪接的保守过程中被移除。虽然在选择的模式生物中得到了很好的研究，但我们才刚刚开始了解整个真核生物树中这一过程的变化和多样性。我们探索了核胚中pre-mRNA剪接和其他转录特征，核胚是次生内共生体高度还原的残核。我们分别对来自红藻和绿藻质体的隐生植物吉拉迪亚（Guillardia theta）和绿蛛形植物Bigelowiella natans进行了链特异性转录组测序。这两种生物都表现出相对于各自细胞核的核形态反义转录和基因表达的升高，表明核形态中基因调控和转录控制的独特特性。在两种核形态之间观察到明显的剪接差异：G. theta核形态的少数内含子在成熟转录本中大部分保留，而B. natans核形态的许多短内含子在典型的真核水平剪接（>90%）。这些剪接水平的差异可能反映了各自质体的祖先，不同的内含子密度由于独立的基因组减少事件，或两者的结合。除了扩展我们对真核生物pre-mRNA剪接多样性的理解外，我们的研究还指出了剪接、反义转录和基因调控之间的潜在联系。
Abstract Eukaryotic genes are interrupted by introns that are removed in a conserved process known as pre-mRNA splicing. Though well-studied in select model organisms, we are only beginning to understand the variation and diversity of this process across the tree of eukaryotes. We explored pre-mRNA splicing and other features of transcription in nucleomorphs, the highly reduced remnant nuclei of secondary endosymbionts. Strand-specific transcriptomes were sequenced from the cryptophyte Guillardia theta and the chlorarachniophyte Bigelowiella natans, whose plastids are derived from red and green algae, respectively. Both organisms exhibited elevated nucleomorph antisense transcription and gene expression relative to their respective nuclei, suggesting unique properties of gene regulation and transcriptional control in nucleomorphs. Marked differences in splicing were observed between the two nucleomorphs: the few introns of the G. theta nucleomorph were largely retained in mature transcripts, whereas the many short introns of the B. natans nucleomorph are spliced at typical eukaryotic levels (>90%). These differences in splicing levels could be reflecting the ancestries of the respective plastids, the different intron densities due to independent genome reduction events, or a combination of both. In addition to extending our understanding of the diversity of pre-mRNA splicing across eukaryotes, our study also indicates potential links between splicing, antisense transcription, and gene regulation in reduced genomes.
已知的、新的和可能的惊喜：对隐植物核型编码蛋白质组的重新评估
影响因子：
3.3
作者：
Zauner S;Heimerl T;Moog D;Maier UG
通讯作者：
Maier UG
影响因子：
64.8
作者：
Curtis, Bruce A.;Tanifuji, Goro;Archibald, John M.
通讯作者：
Archibald, John M.
影响因子：
64.8
作者：
Douglas, S;Zauner, S;Maier, UG
通讯作者：
Maier, UG