Larvicidal activity of saponin from Achyranthes aspera against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)
Larvicidal activity of saponin from Achyranthes aspera against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)
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DOI：
10.1007/s00436-008-0962-z
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发表时间：
2008-06-01
影响因子：
2
通讯作者：
Geetha, Kannappan
中科院分区：
文献类型：
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作者：
Bagavan, A.;Rahuman, A. A.;Geetha, Kannappan
用丙酮、氯仿、乙酸乙酯、正己烷和甲醇5种溶剂对铁苋菜、土牛膝、银合欢、巴戟天和圣罗勒的叶提取物进行了抗埃及伊蚊（Aedes aegypti L）和致倦库蚊（Culex quinquefasciatus Say）4龄初幼虫的活性测定。24 h后观察幼虫死亡率。所有提取物均表现出中等程度的杀幼虫作用，但以乙酸乙酯提取物的幼虫死亡率最高。阿斯佩拉。在本研究中，生物测定引导的A。结果表明，分离鉴定出一种具有杀蚊幼虫潜力的皂苷类化合物，其LC_（50）值分别为18.20和27.24ppm。aegypti和C.致倦肌1H-1 NMR、1C-13 NMR和质谱数据证实了活性化合物的鉴定。这是首次报道从白纹伊蚊乙酸乙酯提取物中提取的皂甙对蚊幼虫的杀灭活性。阿斯佩拉。本研究调查了印度常用草药的粗提物作为控制登革热和淋巴丝虫病媒介的环境安全措施的潜力。
The acetone, chloroform, ethyl acetate, hexane and methanol leaf extracts of Acalypha indica, Achyranthes aspera, Leucas aspera, Morinda tinctoria and Ocimum sanctum were studied against the early fourth-instar larvae of Aedes aegypti L and Culex quinquefasciatus Say. The larval mortality was observed after 24 h exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in the ethyl acetate extract of A. aspera. In the present study, bioassay-guided fractionation of A. aspera led to the separation and identification of a saponin as a potential mosquito larvicidal compound, with LC50 value of 18.20 and 27.24 ppm against A. aegypti and C. quinquefasciatus, respectively. H-1 NMR, C-13 NMR and mass spectral data confirmed the identification of the active compound. This is the first report on the mosquito larvicidal activity of the saponin from the ethyl acetate extract of A. aspera. This study investigates the potential of crude extracts from commonly used medical herbs in India as an environmentally safe measure to control the vector of dengue and lymphatic filariasis.