Workshop and panel discussion: high-throughput cryopreservation of germplasm as an exchange currency for genetic resources.
Workshop and panel discussion: high-throughput cryopreservation of germplasm as an exchange currency for genetic resources.
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研讨会和小组讨论：种质高通量低温保存作为遗传资源的交换货币。
DOI：
10.1016/j.cbpc.2011.06.008
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Tiersch,TerrenceR
中科院分区：
文献类型：
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作者：
Varga,ZoltánM;Tiersch,TerrenceR
在俄勒冈州科瓦利斯举行的第五届人类疾病水生动物模型会议（2010 年 9 月 20 日至 22 日）期间，举办了一次研讨会和小组讨论，以满足水生研究界对种质保存的需求，题为“种质高通量冷冻保存作为遗传资源的交换货币”。青鳉鱼、剑鱼、斑马鱼种群中心的研究人员和代表出席并参与讨论。该研讨会旨在通过评估当前设施、设备和协议方面的需求和机会之间的联系，探索成功的种质库方法（例如牲畜）向水生物种的转移。使用鱼类作为模式生物的生物医学研究已经产生了数以千计的突变体和转基因动物——一种代表巨大科学和信息价值的遗传资源。当信息也可以在生物体中进行测试、操作和研究时，就会增加附加价值，即效用价值。近年来，青鳉鱼、斑马鱼和剑鱼已被成功用作实验室模型生物和研究生物医学问题的工具。例如，剑尾属近交系有助于研究黑色素瘤的原因和病程。青鳉鱼和斑马鱼已被用于遗传筛选，并且已经生产和研究了数千种突变体和转基因品系。因此，生物医学鱼类研究以前所未有的速度产生遗传资源。这些资源的利用价值现在越来越受到维持鱼类种群存活的限制，并且存在许多（如果不是大部分）可能消失的潜在危险。在这种情况下，种质可以被视为第三种价值形式，一种交换货币，允许遗传资源的信息和效用价值的创造、维护、运输和交换。种质的冷冻保存还可以更好地规划数十年的大型研究项目的各个阶段，例如遗传筛选。
A workshop and panel discussion to address the needs of the aquatic research community for germplasm conservation was held during the 5th Aquatic Animal Models for Human Disease Conference in Corvallis, Oregon (September 20–22, 2010), entitled Highthroughput Cryopreservation of Germplasm as an Exchange Currency for Genetic Resources. Researchers and representatives of medaka, Xiphophorus, and zebrafish Stock Centers were present and participated in the discussion. The workshop aimed at exploring the transfer of successful germplasm banking approaches (eg in livestock) to aquatic species, by evaluating linkages across current needs and opportunities in facilities, equipment, and protocols.Biomedical research using fish as model organisms has produced thousands of mutants and transgenic animals—a genetic resource that represents an enormous scientific and informational value. Additional value, utility value, is added when the information can also be tested, manipulated, and studied in living organisms. In recent years medaka, zebrafish, and Xiphophorus have been used with great success as laboratory model organisms and as tools to study biomedical questions. Inbred Xiphophorus lines for example, have been instrumental to study the causes and course of melanomas. Medaka and zebrafish have been used in genetic screens and thousands of mutants and transgenic strains have been produced and studied. As such, research in biomedical fishes generates genetic resources at an unprecedented rate. The utility value of these resources is now increasingly limited by the constraints of maintaining the fish strains alive, and there is potential danger that many if not most of them could be lost. In this scenario, germplasm can be viewed as a third form of value, an exchange currency that allows creation, maintenance, transport, and exchange of the informational and utility values of genetic resources. Cryopreservation of germplasm also allows better planning of phases of large-scale research projects over the course of decades, such as genetic screens.