Sex and housing conditions affect the 24-h acetylcholine release profile in the hippocampus in rats
Sex and housing conditions affect the 24-h acetylcholine release profile in the hippocampus in rats
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DOI：
10.1016/j.neuroscience.2005.01.010
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Kimura, F
中科院分区：
文献类型：
--
作者：
Masuda, J;Mitsushima, D;Kimura, F
为了研究海马中乙酰胆碱（ACh）释放的24小时曲线的性别差异，除了住房条件对该曲线的影响外，我们在生活在大（直径=35 cm）或小（直径=19 cm）圆柱形笼中的完整雄性和循环雌性大鼠中进行了体内微透析研究。将每只大鼠单独圈养在笼中4天。在实验当天，在自由移动条件下超过24 h，以20 min的间隔从背侧海马收集透析液，并以2 h的间隔同时获得连续的血液样品。用高效液相色谱法测定透析液中ACh的含量，用放射立体分析法测定血清中皮质酮的含量。虽然ACh的释放在两种性别的大鼠中均显示出明显的日节律，但雌性大鼠的ACh释放量显著低于雄性大鼠。此外，在小笼子中的住房显着衰减ACh释放在黑暗期的雄性大鼠，但不是在雌性大鼠。相反，血清皮质酮浓度表现出明显的日节律，雌性大鼠血清皮质酮的平均浓度显著高于雄性大鼠。饲养在小笼中不影响两种性别的皮质酮节律。这些结果揭示了性别差异的24小时档案的乙酰胆碱释放，这表明在雄性大鼠的胆碱能系统的脆弱性取决于其住房条件。(c)2005由Elsevier Ltd代表IBRO出版。
To examine the sex difference in the 24-h profile of the acetylcholine (ACh) release in the hippocampus, in addition to the effects of housing conditions on this profile, we performed an in vivo microdialysis study in intact male and cycling female rats that had been living in large (diameter=35 cm) or small (diameter=19 cm) cylindrical cages. Each rat was individually housed in a cage for 4 days. On the day of the experiment, the dialysate was collected from the dorsal hippocampus at 20-min intervals and sequential blood samples were simultaneously obtained at 2-h intervals, under the freely moving condition for more than 24 h. ACh in the dialysates was measured by the high performance liquid chromatography system, while the corticosterone concentration in the serum was measured by radiostereoassay. Although the ACh release showed a clear daily rhythm in both sexes of rats, the amount of ACh released in female rats was significantly lower than that in males. Furthermore, the housing in the small cage significantly attenuated the ACh release during the dark phase in male rats, but not in female rats. Conversely, the serum corticosterone concentration showed a clear daily rhythm and the mean concentration of serum corticosterone in female rats was significantly higher than that in male rats. Housing in the small cage did not affect the corticosterone rhythm in either sex. These results reveal a sex difference in the 24-h profile of the ACh release, which suggests vulnerability of the cholinergic system in male rats depending on its housing conditions. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.