Association of Cdk2/cyclin E and NF-κB complexes at G1/S phase
Association of Cdk2/cyclin E and NF-κB complexes at G1/S phase
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DOI：
10.1006/bbrc.1998.9224
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发表时间：
1998-08-28
影响因子：
3.1
通讯作者：
Li, CCH
中科院分区：
文献类型：
--
作者：
Chen, EY;Li, CCH
NF-κ B/Rel家族在细胞生长、发育、凋亡和应激反应等多种细胞功能中起着关键作用。最近的研究表明，NF-κ B也参与细胞周期调节，并且c-Rel的高表达导致细胞周期停滞在G1/S-期转变（Bash，J.，Zong，W.- X.，和Gelinas，C. 04 The Dog（1997）Cell. 17，6526-6536）。在这里，我们报告的检测Cdk 2，一个关键的激酶负责的G1/S-相转变，在免疫复合物沉淀的NF-κ B抗血清。Cdk 2和NF-κ B B的协会检测共沉淀在G1/S期细胞的核裂解物中，并发现在培养的细胞系和从人外周血中纯化的T细胞。使用含有人c-Rel的C-末端肽的亲和柱，我们分离出细胞周期蛋白E，Cdk 2复合物的调节亚基，作为c-Rel结合蛋白。这些发现支持并提供了NF-κ B参与G1/S期细胞周期控制的物理基础，并提示c-Rel的C端序列发挥重要作用。
NF-kappa B/Rel family plays a pivotal role in a wide variety of cellular functions including growth, development, apoptosis and stress responses. Recent studies indicated that NF-kappa B is also involved in the cell cycle regulation, and high expression of c-Rel results in a cell cycle arrest at the G1/S-phase transition (Bash, J., Zong, W.-X., and Gelinas, C. (1997) Mel. Cell. Biol. 17, 6526-6536). Here we report the detection of Cdk2, a critical kinase responsible for the G1/S-phase transition, in immune complexes precipitated by the NF-kappa B antisera. Cdk2 and NF-kappa B association was detected by co-precipitation in the nuclear lysates of the G1/S-phase cells, and was found in cultured cell lines and in T cells purified from human peripheral blood. Using an affinity column containing the C-terminal peptide of human c-Rel, we isolated cyclin E, the regulatory subunit of the Cdk2 complex, as a c-Rel-binding protein. These findings support and provide physical basis for the involvement of NF-kappa B in the G1/S-phase cell cycle control, and suggest an important role played by the C-terminal sequence of c-Rel.