Cytokine production by allergen (Der pI)-specific CD4+ T cell clones derived from a patient with severe atopic disease
Cytokine production by allergen (Der pI)-specific CD4+ T cell clones derived from a patient with severe atopic disease
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来自患有严重特应性疾病的患者的过敏原 (Der pI) 特异性 CD4 T 细胞克隆产生细胞因子
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
S. Romagnani
中科院分区：
文献类型：
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作者：
P. Parronchi;R. Manetti;C. Simonelli;Francesco Santoni Rugiu;M. Piccinni;E. Maggi;S. Romagnani
摘要从患有严重特应性疾病的患者的外周血中建立了 24 个对纯化的屋尘螨 I 类过敏原 (Der pI) 特异的 T 细胞克隆 (TCC)，并评估了佛波醇 12-肉豆蔻酸酯 13-乙酸酯和抗 CD3 单克隆抗体刺激后细胞因子的产生。所有 Der pI 特异性 TCC 均产生大量与 IL-3、IL-5 和粒细胞单核细胞集落刺激因子 (GM-CSF) 相关的白细胞介素 (IL)-4，而它们产生不同量的 IL-2，并且几乎不产生干扰素-γ。这些数据支持这样的假设：特应性与 2 型 T 辅助细胞的优先激活有关，并表明患有严重特应性疾病的受试者中 IL-3、IL-4、IL-5 和 GM-CSF 基因簇的功能失调。
SummaryTwenty-four T cell clones (TCC) specific for purifiedDermatophagoides pteronyssinus group I allergen (Der pI) were established from the peripheral blood of a patient with severe atopic disease and assessed for cytokine production in response to stimulation with both phorbol 12-myristate 13-acetate and anti-CD3 monoclonal antibody. All Der pI-specific TCC produced high amounts of interleukin (IL)-4 in association with IL-3, IL-5 and granulocyte monocyte-colony stimulating factor (GM-CSF), whereas they produced variable amounts of IL-2 and virtually no interferon-γ. These data support the hypothesis that atopy is associated with preferential activation of type 2 T helper cells and suggest a deregulation in the function of the IL-3, IL-4, IL-5 and GM-CSF gene cluster in subjects with severe atopic disorders.
DOI：
10.1073/pnas.88.10.4538
发表时间：
1991-05-01
影响因子：
11.1
作者：
PARRONCHI, P;MACCHIA, D;ROMAGNANI, S
通讯作者：
ROMAGNANI, S