THE EFFECTS OF TEMPERATURE AND ABA ON STOMATA OF ZEA-MAYS-L
THE EFFECTS OF TEMPERATURE AND ABA ON STOMATA OF ZEA-MAYS-L
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DOI：
10.1093/jxb/33.5.977
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发表时间：
1982-01-01
影响因子：
6.9
通讯作者：
DAVIES, WJ
中科院分区：
文献类型：
--
作者：
RODRIGUEZ, JL;DAVIES, WJ
通过平行于脉撕裂从玉米叶去除表皮碎片。这些细胞在几种浓度的阿坝[脱落酸]中在不同温度下孵育。气孔对温度的敏感性取决于表皮培养技术。通常，最宽的孔径记录在λ。25.degree. C.在所有实验中，气孔在低（10 ℃）下孵育。C）温度为5.6 ×10-4阿坝表现出更宽的孔径比在蒸馏水上孵育。ABA刺激气孔开放的同时，保卫细胞K染色强度增加。在25度。C，在几种浓度的阿坝处理下，表皮气孔关闭，保卫细胞K染色减少，附属细胞K染色增加。
Epidermal fragments were removed from maize leaves by tearing parallel to the veins. These were incubated at a number of different temperatures in several concentrations of ABA [abscisic acid]. The sensitivity of stomata to temperature was dependent upon the technique used to incubate epidermis. Generally, the widest apertures were recorded at .apprx. 25.degree. C. In all experiments, stomata incubated at low (10.degree. C) temperatures on 5.6 .times. 10-4 M ABA showed wider apertures than those incubated on distilled water. This ABA-stimulated stomatal opening was accompanied by an increase in the intensity of K staining in the guard cells. At 25.degree. C, epidermis incubated on several concentrations of ABA showed some stomatal closure, decreased K staining in the guard cells and increased K staining in the subsidiary cells.