NEONATAL HYPERTHYROIDISM IMPAIRS EPINEPHRINE-PROVOKED SECRETION OF NERVE GROWTH-FACTOR AND EPIDERMAL GROWTH-FACTOR IN MOUSE SALIVA
NEONATAL HYPERTHYROIDISM IMPAIRS EPINEPHRINE-PROVOKED SECRETION OF NERVE GROWTH-FACTOR AND EPIDERMAL GROWTH-FACTOR IN MOUSE SALIVA
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DOI：
10.1203/00006450-198607000-00002
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发表时间：
1986-07-01
影响因子：
3.6
通讯作者：
LANDEL, CP
中科院分区：
文献类型：
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作者：
LAKSHMANAN, J;LANDEL, CP
我们研究了新生甲亢对出生31天的雄性和雌性小鼠唾液神经生长因子和表皮生长因子分泌的长期影响。甲状腺功能亢进症(T4)在0-6天内注射(0.4微克/克体重/天)诱发。用赋形剂处理产仔对照组小鼠。T4治疗没有改变分泌到唾液中的蛋白质的数量，但激素治疗引起了分泌蛋白质类型的改变。T4治疗可降低唾液中神经生长因子和表皮生长因子的含量。Sephadex G-200柱层析显示存在两个不同的神经生长因子免疫反应峰，而表皮生长因子免疫反应主要以单一低分子形式洗脱。T4处理不改变其分泌物的分子性质，但处理降低了它们的含量。这些结果表明，在T4治疗停止很长时间后，唾液中神经生长因子和表皮生长因子的分泌受到了损害。
We examined long-term effects of neonatal hyperthyroidism on salivary secretions of nerve growth factor and epidermal growth factor in male and female mice at the age of 31 days. Hyperthyroidism was induced by thyroxine (T4) injections of (0.4 .mu.g/g body weight/day) during days 0-6. Littermate control mice were treated with vehicle. T4 treatment did not alter the amounts of protein secreted into saliva, but hormone administration induced alteration in the types of protein secreted. T4 treatment decreased the contents of both nerve growth factor and epidermal growth factor secreted into the saliva. A Sephadex G-200 column chromatographic profile revealed the presence of two distinct nerve growth factor immunoreactive peaks, while epidermal growth factor immunoreactivity predominantly eluted as a single low molecular weight form. T4 treatment did not alter the molecular nature of their secretion, but the treatment decreased their contents. These results indicate an impairment in salivary secretion of nerve growth factor and epidermal growth factor long after T4 treatment has been discontinued.