Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis
Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis
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中国明对虾雄性特异表达的 CHH 样基因的两种亚型的克隆和表达谱
DOI：
10.1016/j.ygcen.2010.03.028
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发表时间：
2010-06-01
影响因子：
2.7
通讯作者：
Xiang, Jianhai
中科院分区：
文献类型：
--
作者：
Li, Shihao;Li, Fuhua;Xiang, Jianhai
从中国对虾（Fenneropenaeus chinensis）中克隆了两个编码甲壳类高血糖激素（CHH）前体同源基因的全长cDNA序列（Fc-CHH1, Fc-CHH2）及其DNA序列。推测其氨基酸序列包含一个信号肽和一个成熟肽。Fc-CHH1和Fc-CHH2的成熟肽具有78%的同源性，但与其他物种的CHH肽的同源性较低（不到40%）。Fc-CHH1和Fc-CHH2蛋白都含有六个高度保守的半胱氨酸残基，这是CHH家族肽的特征。通过逆转录聚合酶链反应（RT-PCR）检测，发现Fc-CHH1和Fc-CHH2转录本特异性存在于成熟雄虾精包囊中。Fc-CHH1和Fc-CHH2转录本在第一次观察到精包囊出现的未成熟阶段（第一次幼虫期115天后）开始出现。原位杂交分析表明，Fc-CHH1和Fc-CHH2转录本位于精包囊内壁上皮细胞上。在Fc-CHH1和Fc-CHH2克隆的DNA序列中，预测的5'侧翼序列转录因子结合位点与先前报道的甲壳类CHH家族基因不同。据我们所知，这些是在雄性虾中特异性表达的新的chh样基因。它们的功能需要进一步研究。(C) 2010爱思唯尔公司版权所有。
Two full-length cDNA sequences (Fc-CHH1, Fc-CHH2) encoding a crustacean hyperglycemic hormone (CHH) precursor homolog and their DNA sequences were cloned from Chinese shrimp Fenneropenaeus chinensis. The deduced amino acid sequences of them are predicted to contain a signal peptide and a mature peptide. The mature peptides of Fc-CHH1 and Fc-CHH2 shared 78% identity, but they showed low identities (less than 40%) to CHH peptides from other species. Both Fc-CHH1 and Fc-CHH2 proteins contain six highly conserved cysteine residues which are characteristic of the CHH family peptides. The transcripts of Fc-CHH1 and Fc-CHH2 were shown to be specifically present in the spermatophore sac of mature male Chinese shrimp through reverse transcription-polymerase chain reaction (RT-PCR) detection. The transcripts of Fc-CHH1 and Fc-CHH2 begin to appear at the immature stage (115 days after the first post-larvae stage) when the spermatophore sac was first observed to be appeared. In situ hybridization analyses showed that Fc-CHH1 and Fc-CHH2 transcripts located at the epithelial cells in the internal wall of the spermatophore sac. In the cloned DNA sequences of Fc-CHH1 and Fc-CHH2, the predicted transcription factor binding sites in the 5' flanking sequences are different from those previously reported for CHH family genes of crustacean. To our knowledge, these are novel CHH-like genes expressed specifically in male shrimp. Their function needs to be further investigated. (C) 2010 Elsevier Inc. All rights reserved.