IgA1 protease from Neisseria gonorrhoeae inhibits TNFα-mediated apoptosis of human monocytic cells
IgA1 protease from Neisseria gonorrhoeae inhibits TNFα-mediated apoptosis of human monocytic cells
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DOI：
10.1016/s0014-5793(00)01478-2
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发表时间：
2000-04-28
期刊：
影响因子：
3.5
通讯作者：
Meyer, TF
中科院分区：
文献类型：
--
作者：
Beck, SC;Meyer, TF
细胞程序性死亡的调控是炎症和感染性疾病病理生理学的共同主题。IgA1蛋白的合成和分泌与奈瑟氏菌的毒力密切相关。在此，我们报道了高纯度的IgA1蛋白水解酶抑制肿瘤坏死因子-α(TNF-α)介导的人单核细胞系U937的凋亡。通过细胞表面磷脂酰丝氨酸暴露和末端转移酶介导的DNA片断末端标记来验证细胞凋亡。有趣的是，IgA1蛋白水解酶？特异性地切割完整细胞表面的肿瘤坏死因子受体II(TNF-RII)，而肿瘤坏死因子-RI不受该酶的影响，因此，抑制肿瘤坏死因子α介导的细胞凋亡可能与其被NEISSERIAL Ig A1蛋白酶特异性切割有关，(C)2000欧洲生化学会联合会。
The modulation of programmed cell death is a common theme in the patho-physiology of inflammation and infectious disease. The synthesis and secretion of an IgA1 protease is strictly associated with virulence of the Neisseria species. Here, we report on the inhibition of tumor necrosis factor alpha (TNF alpha)-mediated apoptosis of the human myelo-monocytic cell line U937 by highly purified IgA1 protease. Apoptosis was verified by the cell surface exposure of phosphatidyl serine and by terminal transferase mediated end-labeling of fragmented DNA. Interestingly, IgA1 protease? specifically cleaved the TNF receptor II (TNF-RII) on the surface of intact cells whereas TNF-RI was not affected bu the enzyme, Therefore, inhibition of TNF alpha-mediated apoptosis might be correlated to specific cleavage of the TNF-RII by neisserial IgA1 protease, (C) 2000 Federation of European Biochemical Societies.