A HUMAN T-LYMPHOBLASTIC CELL-LINE LACKS LAMIN-A AND LAMIN-C
A HUMAN T-LYMPHOBLASTIC CELL-LINE LACKS LAMIN-A AND LAMIN-C
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DOI：
10.1002/j.1460-2075.1987.tb02715.x
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发表时间：
1987-12-01
期刊：
影响因子：
11.4
通讯作者：
COURVALIN, JC
中科院分区：
文献类型：
--
作者：
GUILLY, MN;BENSUSSAN, A;COURVALIN, JC
层蛋白A、层蛋白B和层蛋白C是核膜的三种主要蛋白质，构成了一类中间丝多肽。我们已经比较了两个人类细胞系--上皮HeLa细胞和T淋巴母细胞KE-37中这些多肽的含量。研究发现，这三种蛋白在HeLa细胞中的化学计量比大致相同，而B蛋白是T淋巴母细胞中唯一的蛋白成分。此外，在HeLa细胞中检测到3kb的层蛋白A和2.1kb的层蛋白C的mRNA，而在T淋巴母细胞中未检测到。这些结果表明：(1)层蛋白B可以建立主动分裂体细胞的层结构；(2)层蛋白在淋巴样细胞中的表达可能受到重要的数量变化的影响。对克隆人T淋巴细胞和EB病毒转化的人B淋巴细胞的层蛋白组成的比较证实了这一说法。两种细胞中层粘连蛋白B的含量基本相同，但层粘连蛋白A和C的含量差异很大，在T细胞中含量较低，在B细胞中含量较高。
Lamins A, B and C, the three major proteins of nuclear envelope, constitute a class of intermediate filament polypeptides. We have compared the amount of these polypeptides in two human cell lines, epithelial HeLa cells and T lymphoblasts KE 37. It was found that the three lamins were present in roughly equimolar stoichiometry in HeLa cells, while lamin B was the unique lamin component in T lymphoblasts. Moreover, 3-kb mRNA of lamin A and 2.1-kb mRNA of lamin C were detected with a human cDNA probe in HeLa cells but not in T lymphoblasts. These results suggest that (i) lamin B can build up the lamina structure in actively dividing somatic cells by itself, and (ii) lamin expression in lymphoid cells may be subject to important quantitative variations. Comparison of the lamin composition of human cloned T lymphocytes and Epstein-Barr virus-transformed human B lymphocytes confirmed this statement. The lamin B level was nearly equivalent in both cells but the content of lamins A and C varied to a large extent, being low in T cells and high in B cells.