Novel broad-spectrum bacteriophages against Xanthomonas oryzae and their biocontrol potential in rice bacterial diseases
Novel broad-spectrum bacteriophages against Xanthomonas oryzae and their biocontrol potential in rice bacterial diseases
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DOI：
10.1111/1462-2920.16447
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发表时间：
2023-06-10
影响因子：
5.1
通讯作者：
Jin，Yi
中科院分区：
文献类型：
--
作者：
Liu，Mengjiao;Hu，Ran;Jin，Yi
细菌性叶枯病（BLB）和细菌性条纹病(BLS) -由水稻黄单胞菌引起。稻瘟病菌（Xoo）和稻瘟病菌。稻瘟病（oryzicola, Xoc）是威胁最重要的粮食作物之一水稻安全生产的两大细菌性病害。由于噬菌体具有寄主特异性和环境安全性，被认为是潜在的水稻细菌性病原体生物防治剂。BLB和BLS在野外同时发生是很常见的，这突出了对能够同时感染xo和Xoc的广谱噬菌体的需求。在本研究中，对两种能感染多种Xoo和Xoc菌株的裂解广谱噬菌体（pXoo2106和pXoo2107）进行了评估。这两种噬菌体都属于尾状病毒科，其中一种属于亲笔病毒科，而另一种属于未分类的科。两个噬菌体单独或结合在噬菌体鸡尾酒中可以有效地抑制Xoo和Xoc的体外生长。在体内生物防治实验中，噬菌体鸡尾酒降低了总CFU，显著缓解了Xoo或Xoc引起的症状。我们的研究结果表明，pXoo2106和pXoo2107具有针对不同x的广谱宿主范围。在田间应用中对BLB和BLS均具有很强的生物防治潜力。
Bacterial leaf blight (BLB) and bacterial leaf streak (BLS)—caused byXanthomonas oryzaepv. oryzae(Xoo) andXanthomonas oryzaepv.oryzicola(Xoc), respectively—are two major bacterial diseases that threaten the safe production of rice, one of the most important food crops. Bacteriophages are considered potential biocontrol agents against rice bacterial pathogens, due to their host specificity and environmental safety. It is common for BLB and BLS to occur together in fields, which highlights the need for broad‐spectrum phages capable of infecting both Xoo and Xoc. In this study, two lytic broad‐spectrum phages (pXoo2106 and pXoo2107) that can infect various strains of Xoo and Xoc were assessed. Both phages belong to the classCaudoviricetesand one of them to the familyAutographiviridae, while the other belongs to an unclassified family. Two phages alone or combined in a phage cocktail could effectively inhibit Xoo and Xoc growth in vitro. In an in vivo biocontrol experiment, the phage cocktail reduced the total CFU and significantly eased the symptoms caused by Xoo or Xoc. Our results suggest that pXoo2106 and pXoo2107 have a broad‐spectrum host range targeting differentX. oryzaestrains, and have strong biocontrol potential in field applications against both BLB and BLS.