Wide-field imaging of birefringent synovial fluid crystals using lens-free polarized microscopy for gout diagnosis.
Wide-field imaging of birefringent synovial fluid crystals using lens-free polarized microscopy for gout diagnosis.
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DOI：
10.1038/srep28793
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发表时间：
2016-06-30
影响因子：
4.6
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Zhang Y;Lee SY;Zhang Y;Furst D;Fitzgerald J;Ozcan A
痛风是晶体关节病的一种形式，尿酸钠 (MSU) 晶体沉积并引起关节炎症。痛风的诊断依赖于在补偿偏光显微镜 (CPLM) 下从患者关节抽吸的滑液中识别 MSU 晶体。 MSU 晶体的双折射特性增强了光学显微镜对 MSU 晶体的检测能力。然而，CPLM 部分受到传统基于透镜的显微镜的高成本和笨重的影响，并且其相对较小的视场 (FOV) 限制了痛风诊断的效率和准确性。在这里，我们推出了一种无透镜偏光显微镜，它采用新颖的差分和角度不匹配的偏光光学设计，可实现双折射物体的宽视场和高分辨率全息成像，其颜色对比度与标准 CPLM 相似。这种计算偏光显微镜的性能通过对由痛风患者的痛风石制成的 MSU 晶体和用作阴性对照的类固醇晶体进行成像来验证。这种无镜头偏光显微镜具有宽视场（>20 mm2）、成本效益和现场便携性，可以显着提高痛风诊断的效率和准确性，降低成本，甚至可以在护理点和资源有限的临床环境中部署。
Gout is a form of crystal arthropathy where monosodium urate (MSU) crystals deposit and elicit inflammation in a joint. Diagnosis of gout relies on identification of MSU crystals under a compensated polarized light microscope (CPLM) in synovial fluid aspirated from the patient’s joint. The detection of MSU crystals by optical microscopy is enhanced by their birefringent properties. However, CPLM partially suffers from the high-cost and bulkiness of conventional lens-based microscopy, and its relatively small field-of-view (FOV) limits the efficiency and accuracy of gout diagnosis. Here we present a lens-free polarized microscope which adopts a novel differential and angle-mismatched polarizing optical design achieving wide-field and high-resolution holographic imaging of birefringent objects with a color contrast similar to that of a standard CPLM. The performance of this computational polarization microscope is validated by imaging MSU crystals made from a gout patient’s tophus and steroid crystals used as negative control. This lens-free polarized microscope, with its wide FOV (>20 mm2), cost-effectiveness and field-portability, can significantly improve the efficiency and accuracy of gout diagnosis, reduce costs, and can be deployed even at the point-of-care and in resource-limited clinical settings.