Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
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DOI：
10.1038/nature01266
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发表时间：
2002-12-05
期刊：
影响因子：
64.8
通讯作者：
Hayashizaki, Y
中科院分区：
文献类型：
--
作者：
Okazaki, Y;Furuno, M;Hayashizaki, Y
只有一小部分小鼠基因组被转录成成熟的信使RNA转录本。有一个国际合作的努力，以确定所有全长mRNA转录的小鼠，并确保每一个代表在一个物理收集的克隆。在这里，我们报告了60，770个全长小鼠互补DNA序列的手动注释。这些被聚类为33，409个“转录单位”，占新建立的小鼠转录组数据库的90.1%。在这些转录单位中，4，258个是新的蛋白质编码，11，665个是新的非编码信息，表明非编码RNA是转录组的主要组成部分。所有转录单位的41%显示出选择性剪接的证据。在蛋白质编码转录本中，79%的剪接变异改变了蛋白质产物。全转录组分析鉴定出2，431个有义-反义对。目前的工作，完全支持物理克隆，提供了最全面的调查哺乳动物转录组到目前为止，是一个有价值的资源，功能基因组学。
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.