Plant DNA-damage repair/toleration 100 protein repairs UV-B-induced DNA damage
Plant DNA-damage repair/toleration 100 protein repairs UV-B-induced DNA damage
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DOI：
10.1016/j.dnarep.2014.05.009
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发表时间：
2014-09-01
期刊：
影响因子：
3.8
通讯作者：
Suzuki, Shunji
中科院分区：
文献类型：
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作者：
Fujimori, Nozomi;Suzuki, Nana;Suzuki, Shunji
我们报道了葡萄DNA损伤修复/耐受100蛋白VvDRT100-L的特性。VvDRT100-L有9个富含亮氨酸的重复序列，属于植物DRT100蛋白家族。VvDRT100-L在葡萄的绿色器官中大量表达，包括卷须、叶片和绿色浆果皮。VvDRT100-L在拟南芥中的过表达降低了UV-B辐射损伤的DNA的基本位点数和DNA单链断裂频率，而UV-B辐射显著增加了T-DNA插入突变体drt100的基本位点数和DNA单链断裂频率。VvDRT100-L-高表达植株在致死剂量的紫外线照射下仍能存活并表现出明显的健康，表明VvDRT100-L可能提高了植物对紫外线的耐受性。综上所述，VvDRT100-L可能在UV-B诱导的DNA损伤的修复和耐受中发挥重要作用。这些发现将有助于我们更好地了解植物如何获得紫外线胁迫适应、耐受性和DNA修复。(C)2014爱思唯尔B.V.保留所有权利。
We report the characterization of VvDRT100-L, a grape DNA-damage repair/toleration 100 protein. VvDRT100-L has nine leucine-rich repeats and belongs to the plant DRT100 protein family. VvDRT100-L is expressed abundantly in green organs of grapevines, including tendrils, leaves, and green berry skins. The overexpression of VvDRT100-L in Arabidopsis plants decreased the number of abasic sites and the frequency of DNA single-strand breaks in the DNA damaged by UV-B irradiation, whereas UV-B irradiation markedly increased the number of abasic sites and the frequency of DNA single-strand breaks in T-DNA insertion mutant drt100 plants. VvDRT100-L-overexpressing plants remained viable and noticeably healthy under lethal UV doses, suggesting that VvDRT100-L may enhance UV tolerance in plant. Taken together, we concluded that VvDRT100-L might play an important role in the repair and toleration of UV-B-induced DNA damage. These findings would help us better understand how plants acquire UV stress acclimation, tolerance and DNA repair. (C) 2014 Elsevier B.V. All rights reserved.