Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution.
Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution.
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DOI：
10.1016/j.cels.2023.08.003
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发表时间：
2023-09
期刊：
影响因子：
9.3
通讯作者：
Hai-Ning Lyu;Chunjin Fu;Xin Chai;Zipeng Gong;Junzhe Zhang;Jiaqi Wang;Jigang Wang;Lingyun Dai;Chengchao Xu
中科院分区：
文献类型：
--
作者：
Hai-Ning Lyu;Chunjin Fu;Xin Chai;Zipeng Gong;Junzhe Zhang;Jiaqi Wang;Jigang Wang;Lingyun Dai;Chengchao Xu
了解植物蛋白质组在天然细胞环境中的热稳定性将有助于设计具有高耐热性的作物，但现有的此类数据有限。在此，我们应用定量质谱仪对拟南芥蛋白质组的热稳定性进行了分析，并鉴定了拟南芥中的热敏感和热弹性蛋白质网络，为理解热诱导损伤提供了基础。我们还表明，蛋白质熔化曲线的相似性可以作为评估非工程植物中系统范围的蛋白质-蛋白质相互作用的指标，并能够在细胞环境的背景下识别代谢物表现出的瞬时相互作用。最后，我们系统地比较了拟南芥中近亲的热稳定性，以帮助研究和理解基因复制和蛋白质进化。综上所述，我们的结果可能会对植物耐热性、植物蛋白组装和进化领域产生广泛的影响。
Understanding the thermal stability of the plant proteome in the context of the native cellular environment would aid the design of crops with high thermal tolerance, but only limited such data are available. Here, we applied quantitative mass spectrometry to profile the thermal stability of theArabidopsisproteome and identify thermo-sensitive and thermo-resilient protein networks inArabidopsis, providing a basis for understanding heat-induced damage. We also show that the similarities of the protein-melting curves can be used as a proxy to evaluate system-wide protein-protein interactions in non-engineered plants and enable the identification of transient interactions exhibited by metabolons in the context of the cellular environment. Finally, we report a systematic comparison of the thermal stability of paralogs inArabidopsisto aid the investigation and understanding of gene duplication and protein evolution. Taken together, our results could have broad implications for the fields of plant thermal tolerance, plant protein assemblies, and evolution.