Importance of viral genomic composition in modulating glycoprotein content on the surface of influenza virus particles
Importance of viral genomic composition in modulating glycoprotein content on the surface of influenza virus particles
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DOI：
10.1016/j.virol.2011.03.011
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发表时间：
2011-05-25
期刊：
影响因子：
3.7
通讯作者：
Lina, Bruno
中科院分区：
文献类型：
--
作者：
Moules, Vincent;Terrier, Olivier;Lina, Bruno
尽管我们对流感病毒颗粒内部组织的认识取得了进展，但对其形态的决定因素，尤其是病毒体水平上结构蛋白的实际丰度知之甚少。为了解决这些问题，我们使用cryo-EM专注于病毒（和宿主）因素，这些因素可能导致观察到的病毒体形态和特征（如大小、形状和糖蛋白（GP）峰密度）差异。12个重组病毒，其特征在于它们的形态，神经氨酸酶活性和病毒生长。基因组组成被证明是重要的，在确定GP穗密度。特别是，聚合酶基因片段，特别是PB 1/PB 2显示出具有显着的影响，除了HA在确定GP峰密度，这些病毒组件之间的功能性联系的功能一致的特征，重要的病毒适应性。(C)2011 Elsevier Inc. All rights reserved.
Despite progress in our knowledge of the internal organisation of influenza virus particles, little is known about the determinants of their morphology and, more particularly, of the actual abundance of structural proteins at the virion level. To address these issues, we used cryo-EM to focus on viral (and host) factors that might account for observed differences in virion morphology and characteristics such as size, shape and glycoprotein (GP) spike density. Twelve recombinant viruses were characterised in terms of their morphology, neuraminidase activity and virus growth. The genomic composition was shown to be important in determining the GP spike density. In particular, polymerase gene segments and especially PB1/PB2 were shown to have a prominent influence in addition to that for HA in determining GP spike density, a feature consistent with a functional link between these virus components important for virus fitness. (C) 2011 Elsevier Inc. All rights reserved.