Isolation and characterisation of lactic acid bacterium for effective fermentation of cellobiose into optically pure homo L-(+)-lactic acid
Isolation and characterisation of lactic acid bacterium for effective fermentation of cellobiose into optically pure homo L-(+)-lactic acid
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DOI：
10.1007/s00253-010-2986-4
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发表时间：
2011-02-01
影响因子：
5
通讯作者：
Sonomoto, Kenji
中科院分区：
文献类型：
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作者：
Abdel-Rahman, Mohamed Ali;Tashiro, Yukihiro;Sonomoto, Kenji
有效利用纤维素生物质以经济地生产乳酸需要具有有效利用其主要组分葡萄糖和纤维二糖的潜在能力的微生物。在从不同环境样品中分离的631株菌株中，菌株QU 25从纤维二糖中产生高产量的高光学纯度的l-（+）-乳酸。根据其糖发酵模式和16 S rDNA序列分析，将QU 25菌株鉴定为蒙氏肠球菌（Enterococcus mundtii）。对E. mundtii QU 25菌株分批培养的最适pH和温度分别为7.0A ℃和43 A ℃。E. mundtii QU 25能够同时代谢葡萄糖和纤维二糖的混合物，而没有明显的碳分解代谢物抑制。此外，在优化的培养条件下，光学纯的L-乳酸（99.9%）的生产随着纤维二糖浓度的增加而增加。这表明E. mundtii QU 25是从纤维素水解物材料有效生产乳酸的潜在候选物。
Effective utilisation of cellulosic biomasses for economical lactic acid production requires a microorganism with potential ability to utilise efficiently its major components, glucose and cellobiose. Amongst 631 strains isolated from different environmental samples, strain QU 25 produced high yields of l-(+)-lactic acid of high optical purity from cellobiose. The QU 25 strain was identified as Enterococcus mundtii based on its sugar fermentation pattern and 16S rDNA sequence. The production of lactate by fermentation was optimised for the E. mundtii QU25 strain. The optimal pH and temperature for batch culturing were found to be 7.0A degrees C and 43A degrees C, respectively. E. mundtii QU 25 was able to metabolise a mixture of glucose and cellobiose simultaneously without apparent carbon catabolite repression. Moreover, under the optimised culture conditions, production of optically pure l-lactic acid (99.9%) increased with increasing cellobiose concentrations. This indicates that E. mundtii QU 25 is a potential candidate for effective lactic acid production from cellulosic hydrolysate materials.