Prognostic Impact of Angiogenesis in Nonmuscle Invasive Bladder Cancer as Defined by Microvessel Density after Immunohistochemical Staining for CD34
Prognostic Impact of Angiogenesis in Nonmuscle Invasive Bladder Cancer as Defined by Microvessel Density after Immunohistochemical Staining for CD34
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DOI：
10.3109/01913123.2012.672847
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发表时间：
2012-10-01
影响因子：
1
通讯作者：
Boubaker, Samir
中科院分区：
文献类型：
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作者：
Ajili, Faouzia;Kacem, Monia;Boubaker, Samir
膀胱癌是泌尿生殖系统第二常见的恶性肿瘤。大多数膀胱癌死亡是转移性疾病的结果。微血管密度（MVD）是血管生成的替代标志物，已被证明可预测疾病进展和不良预后。本研究的目的是评估血管生成在BCG免疫治疗的人非肌层浸润性膀胱癌（NMIBC）中的预测价值和预后意义。应用免疫组化SP法对28例非肌层浸润性膀胱癌冰冻切片进行CD34标记。血管生成活性通过血管标记物CD34的表达测定微血管计数来测量。采用单因素和多因素分析方法研究了肿瘤分期、分级、位点数、肿瘤大小、年龄和CD 34表达在确定复发风险中的预后意义。根据对NMIBC患者的肿瘤分期、分级、肿瘤大小、位点数、年龄和CD34表达的预后意义的单变量分析，pT1分期和高分级似乎以统计学显著的方式与较高的复发风险相关（分别为P=0.004，P=0.004）。另一方面，多因素考克斯回归分析显示，微血管密度和多样性是BCG免疫治疗后复发的独立预测因子（分别为p=0.016，p=0.032）。本研究为CD34 MVD与BCG免疫治疗后复发相关提供了强有力的证据。然而，需要对更大的队列进行独立研究来验证这些发现。
Bladder cancer is the second most common malignancy of the urogenital region. The majority of bladder cancer deaths occur as a consequence of metastatic disease. Microvessel density (MVD), a surrogate marker for angiogenesis, has been shown to be predictive of progression and poor prognosis. The aim of this study was to evaluate the predictive value and prognostic significance of angiogenesis in human non muscle invasive bladder cancer (NMIBC) treated by BCG immunotherapy. The frozen sections of 28 non muscle invasive bladder cancer specimens were stained with CD34 antibody to label the vascular endothelium using the standard streptavidinbiotin immunoperoxidase method. Angiogenic activity was measured using microvessel count determined by the expression of vascular markers CD34. The prognostic significance of tumor stage, grade, loci number, tumor size, age and CD34 expression in determining the risk for recurrence was studied with both univariate and multivariate methods of analysis. According to univariate analysis of the prognostic significance for tumor stage, grade, tumor size, loci number, age and CD34 expression, in patients with NMIBC, the pT1 stage and high grade seem to be associated in a statistically significant manner with higher risk for recurrence (P=0.004, P=0.004, respectively). In the other hand, multivariate Cox regression's analysis showed that microvessel density and multiplicity were independent predictor of recurrence after BCG immunotherapy (p=0.016, p=0.032, respectively). This study provides strong evidence that CD34 MVD is associated with recurrence after BCG immunotherapy. Independent studies, however, will be required on larger cohort to validate these findings.