Algorithm for accurate similarity measurements of peptide mass fingerprints and its application
Algorithm for accurate similarity measurements of peptide mass fingerprints and its application
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DOI：
10.1016/j.jasms.2004.09.013
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发表时间：
2005-01-01
影响因子：
3.2
通讯作者：
Berndt, P
中科院分区：
文献类型：
--
作者：
Monigatti, F;Berndt, P
我们提出了一个简单的算法，它允许准确估计的相似性肽指纹质谱从基质辅助激光解吸/电离（MALDI）光谱仪。该算法是质量相关性和强度等级相关性的组合，用于聚类相似的光谱，并从超过100，000个光谱的数据存储中生成一致的光谱。由此产生的第一个光谱库的1248明确确定不同的蛋白质酶被用来搜索错过的切割模式，迄今为止还没有报道，并揭示了一些肽电离特性。这项研究的结果可以直接应用于肽质量指纹搜索算法，以降低假阳性错误率，
We present a simple algorithm which allows accurate estimates of the similarity between peptide fingerprint mass spectra from matrix assisted laser desorption/ionization (MALDI) spectrometers. The algorithm, which is a combination of mass correlation and intensity rank correlation, was used to cluster similar spectra and to generate consensus spectra from a data store of more than 100,000 spectra. The resulting first spectra library of 1248 unambiguously identified different protein digests was used to search for missed cleavage patterns that have not been reported so far and to shed light on some peptide ionization characteristics. The findings of this study could be directly implemented in peptide mass fingerprint search algorithms to decrease the false positive error rate to