Demonstration of viable Chlamydia pneumoniae in atherosclerotic plaques of carotid arteries by reverse transcriptase polymerase chain reaction
Demonstration of viable Chlamydia pneumoniae in atherosclerotic plaques of carotid arteries by reverse transcriptase polymerase chain reaction
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DOI：
10.1007/s100169900277
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发表时间：
1999-07-01
影响因子：
1.5
通讯作者：
Fagetti, L
中科院分区：
文献类型：
--
作者：
Esposito, G;Blasi, F;Fagetti, L
已有多项研究证实动脉粥样硬化中肺炎衣原体的存在。从动脉组织中培养这种微生物一直很困难。我们报告使用逆转录聚合酶链式反应来检测颈动脉粥样硬化中肺炎衣原体的存活。我们分析了30例(女性14例，平均年龄69.6+/-8.8岁)接受颈动脉粥样硬化斑块摘除手术的患者。术中取舌静脉和甲状上动脉标本。我们将两种分子生物学技术应用于舌静脉或甲状腺动脉标本上的颈动脉斑块：1)聚合酶链式反应(PCR)和2)逆转录-聚合酶链式反应(RT-PCR)检测细菌mRNA，使用设计的检测16S rRNA基因片段的聚合酶链式反应(PCR)引物。用微量免疫荧光法测定肺炎衣原体抗体效价。本研究的结果证实了动脉粥样硬化中肺炎衣原体活性的存在，并支持该生物体在动脉粥样硬化发病机制中可能是一个活跃因素的假说。
The presence of Chlamydia pneumoniae in atheromas has been demonstrated in several studies. Culture of the organism from arterial tissue has been difficult. We report the use of a reverse transcriptase polymerase chain reaction to detect viable Chlamydia pneumoniae in carotid atheromas. We analyzed 30 patients (14 females, mean age 69.6 +/- 8.8 years) who underwent surgery for the removal of atherosclerotic plaques from carotid arteries. During surgery, samples of lingual vein and superior thyroideal artery were also taken. We applied two molecular biology techniques to the carotid plaques on lingual vein or thyroideal artery samples: 1) polymerase chain reaction (PCR) and 2) reverse transcriptase-PCR (RT-PCR) for the detection of bacterial mRNA, employing PCR primers designed to detect a fragment of the 16S rRNA gene. Blood samples were obtained from the patients for determination of Chlamydia pneumoniae IgG, IgA, and IgM antibody titers by a microimmunofluorescence technique. The results of the present study confirmed the presence of viable Chlamydia pneumoniae in atheromas and support the hypothesis that the organism may be an active factor in the pathogenesis of atherosclerosis.