DNA sequences coding for the F18 fimbriae and AIDA adhesin are localised on the same plasmid in Escherichia coli isolates from piglets
DNA sequences coding for the F18 fimbriae and AIDA adhesin are localised on the same plasmid in Escherichia coli isolates from piglets
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DOI：
10.1016/s0378-1135(02)00019-6
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发表时间：
2002-05-24
影响因子：
3.3
通讯作者：
Benz, I
中科院分区：
文献类型：
--
作者：
Mainil, JG;Jacquemin, E;Benz, I
粘附素参与弥漫性粘附（AIDA）无菌毛粘附素由人大肠杆菌产生，但动物大肠杆菌不产生，德国猪致Vero细胞性大肠杆菌（VTEC）除外[Clin. Diagn. Lab. Immunol.8（2001）143]。在比利时VTEC和非VTEC大肠杆菌的集合中研究了编码和产生AIDA粘附素的DNA序列（aidA）的存在和定位。仔猪断奶时分离的大肠杆菌。还通过菌落杂交研究了174个分离株中是否存在编码Stx2e verocytoxin和F18菌毛粘附素（fed）的DNA序列：71个为Stx2 + F18 + AIDA+，26个为F18 + AIDA+，12个为AIDA+，2个为Stx2 + AIDA+，1个仅为Stx2+。58株（F18+）AIDA+分离株中有54株在免疫印迹试验中检测为阳性，免疫血清针对AIDA蛋白。与质粒DNA图谱上的AIDA gone探针杂交，在10个AIDA+和25个研究的F18 + AIDA+分离株中的24个中鉴定出探针阳性血浆条带。此外，在F18 + AIDA+分离物中，只有一条质粒带与F18和AIDA探针杂交。这些结果证实了aidA相关基因不仅存在于来自仔猪的VTEC分离物中，而且存在于来自仔猪的非VTEC分离物中，并且大多数探针阳性的E.杆菌此外，质粒DNA杂交结果表明aidA和fed相关DNA序列定位在同一质粒上。(C)2002 Elsevier Science B.V.保留所有权利。
The adhesin-involved-in-diffuse-adherence (AIDA) afimbrial adhesin is produced by human, but not by animal, Escherichia coli, with the exception of German porcine verotoxigenic Escherichia coli (VTEC) [Clin. Diagn. Lab. Immunol. 8 (2001) 143]. Presence and localisation of DNA sequences (aidA) coding for and production of an AIDA adhesin were investigated in a collection of Belgian VTEC and non-VTEC E. coli isolated from piglets at weaning time. The 174 isolates were also studied by colony hybridisation for the presence of DNA sequences coding for the Stx2e verocytotoxin and the F18 fimbrial adhesin (fed): 71 were Stx2 + F18 + AIDA+, 26 were F18 + AIDA+, 12 were AIDA+, two were Stx2 + AIDA+, and one was Stx2+ only. Fifty-four of the 58 (F18+)AIDA+ isolates tested positive in a western blotting assay with an immune serum raised against the AIDA protein. Hybridisation with the AIDA gone probe on plasmid DNA profiles identified a probe-positive plasma band in the 10 AIDA+ and in 24 of the 25 F18 + AIDA+ isolates studied. Moreover in F18 + AIDA+ isolates, only one plasmid band hybridised with both F18 and AIDA probes, These results confirm the presence of aidA-related genes in not only VTEC, but also non-VTEC, isolates from piglets and the production of an antigenically AIDA-related protein by the majority of probe-positive E. coli. Moreover the plasmid DNA hybridisation results suggest a localisation on the same plasmid of the aidA- and fed-related DNA sequences. (C) 2002 Elsevier Science B.V. All rights reserved.