Development and characterization of EST-SSR markers in Asarum sakawanum var. stellatum and cross-amplification in related species
Development and characterization of EST-SSR markers in Asarum sakawanum var. stellatum and cross-amplification in related species
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细辛 EST-SSR 标记的开发和鉴定
DOI：
10.1111/1442-1984.12156
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发表时间：
2017
影响因子：
1.4
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Takahashi;D.;Sakaguchi;S.;Setoguchi;H.
建立了细辛的EST - SSR标记。本研究旨在对asarum属其他系列asarum的遗传多样性和遗传结构进行评价。基于转录组数据，共设计了1864对引物，包括1864个三核苷酸SSR基序。在96个引物中，17个位点成功扩增并在seriesSakawanumtaxa中多态性。此外，所有这些基因座都成功扩增到inA。而在系统发育较远的a株中，只有10个基因座被扩增。pellucidum。对于a的人口。sakawanumvar。星状体的观察杂合度和期望杂合度分别为0.261 ~ 0.957和0.317 ~ 0.881。这些新开发的EST - SSR标记将用于akawanumseries的群体遗传研究，以推断这些系列的进化历史和遗传多样性，其中许多在日本处于濒危状态。
EST‐SSR markers were developed forAsarum sakawanumvar.stellatumto evaluate the genetic diversity and genetic structure of seriesSakawanumand assess their utility in other series in the genusAsarum. A total of 1864 primer pairs, including 1864 tri‐nucleotide SSR motifs, were designed based on transcriptome data. Of the 96 primers tested, 17 loci were successfully amplified and polymorphic in seriesSakawanumtaxa. Furthermore, all these loci were successfully amplified inA. takaoi, whereas only 10 loci were amplified in the more phylogenetically distantA. pellucidum. For populations ofA. sakawanumvar.stellatum, the values of observed and expected heterozygosity ranged from 0.261 to 0.957 and 0.317 to 0.881, respectively. These newly developed EST‐SSR markers will be used in population genetic studies of seriesSakawanumto infer the evolutionary history and genetic diversity of these series, many of which are endangered in Japan.