A malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, regulates powdery mildew resistance in wheat
A malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, regulates powdery mildew resistance in wheat
复制标题
类malectin/富含亮氨酸重复受体蛋白激酶基因RLK-V调节小麦白粉病抗性
DOI：
10.1111/mpp.12729
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发表时间：
2018-12-01
影响因子：
4.9
通讯作者：
Cao, Aizhong
中科院分区：
文献类型：
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作者：
Hu, Ping;Liu, Jiaqian;Cao, Aizhong
模式识别受体（PRR）可以通过识别病原体相关的分子模式来触发植物免疫。在这项研究中，我们报道了来自 Haynaldia villosa 的类似 malectin/富含亮氨酸的重复受体蛋白激酶基因 RLK-V，推测它可以作为 PRR 来积极调节由 Blumeria graminis f 引起的白粉病抗性。 sp。小麦中的小麦（Bgt）。 RLK-V 有两个选择性剪接的转录本，对应于完整的 RLK-V1.1 和由内含子保留引起的截短的 RLK-V1.2。表达分析表明，在抗性材料中，两个转录本均可被 Bgt 上调，而功能性 RLK-V1.1 仅在接种 Bgt 后才表达。启动子活性测定表明，RLK-V 即使在易感小麦中也能响应 Bgt。携带 Pm21 的抗性材料中 RLK-V 的沉默导致对 Bgt 的抗性受损。此外，通过在扬麦158中单细胞瞬时表达和稳定转化，在Pm21缺乏的感病扬麦158和SM-1中过量表达RLK-V1.1可以提高白粉病抗性。我们认为 RLK-V 调节对白粉病的基础抗性，这也是 Pm21 基因介导的广谱抗性所必需的。 RLK-V1.1的过度表达可以引发本塞姆氏烟草的细胞死亡，并且RLK-V1.1转基因小麦比受体积累更多的活性氧并表现出更强的过敏反应，从而导致Bgt抗性增强。然而，RLK-V1.1的组成型激活导致转基因植物生长异常。
Pattern recognition receptors (PRRs) can trigger plant immunity through the recognition of pathogen-associated molecular patterns. In this study, we report that a malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, from Haynaldia villosa putatively acts as a PRR to positively regulate powdery mildew resistance caused by Blumeria graminis f. sp. tritici (Bgt) in wheat. RLK-V has two alternatively spliced transcripts corresponding to an intact RLK-V1.1 and a truncated RLK-V1.2 caused by intron retention. Expression analysis showed that both transcripts could be up-regulated by Bgt in resistant materials, whereas the functional RLK-V1.1 was expressed only after Bgt inoculation. Promoter activity assays indicated that RLK-V could respond to Bgt even in susceptible wheat. Silencing of RLK-V in Pm21-carrying resistant materials resulted in compromised resistance to Bgt. In addition, over-expression of RLK-V1.1 in Pm21-lacking susceptible Yangmai158 and SM-1 by single-cell transient expression and stable transformation in Yangmai158 could improve powdery mildew resistance. We propose that RLK-V regulates basal resistance to powdery mildew, which is also required for broad-spectrum resistance mediated by the Pm21 gene. Over-expression of RLK-V1.1 could trigger cell death in Nicotiana benthamiana, and RLK-V1.1 transgenic wheat accumulated more reactive oxygen species and displayed a stronger hypersensitive response than did the recipient, which led to enhanced Bgt resistance. However, constitutive activation of RLK-V1.1 resulted in the abnormal growth of transgenic plants.