Expression of estrogen receptor-β messenger ribonucleic acid in oxytocin and vasopressin neurons of the rat supraoptic and paraventricular nuclei
Expression of estrogen receptor-β messenger ribonucleic acid in oxytocin and vasopressin neurons of the rat supraoptic and paraventricular nuclei
复制标题
DOI：
10.1210/en.139.5.2600
复制
发表时间：
1998-05-01
期刊：
影响因子：
4.8
通讯作者：
Liposits, Z
中科院分区：
文献类型：
--
作者：
Hrabovszky, E;Kalló, I;Liposits, Z
雌激素对室旁核(PVN)和视上核(SON)大细胞催产素(OT)和加压素(VP)神经元的调节作用已有文献报道。到目前为止，雌激素的作用是直接发挥作用还是通过雌激素敏感的中间神经元发挥作用仍存在争议。以前的免疫细胞化学(ICC)和原位杂交(ISH)研究发现，在大鼠的PVN和SON中，经典雌激素受体(ER-α)要么是合法水平的，要么是缺失的。本实验采用ICC和ISH相结合的方法，观察了新近克隆的雌激素受体(ER-β)在大鼠下丘脑OT和VP免疫反应(IR)神经元系统中的表达。结果表明，OT-IR神经元中ER-β信使RNA表达水平最高的部位是下丘脑室旁核的尾侧和TTP-ITC细胞中央核的腹内侧区。这些神经元以前被证明投射到脑干和脊髓的尾部，以调节自主神经功能。此外，PVN和SON的整个吻尾部都含有共同表达不同水平ER-βmRNA的OT-IR神经元。类似地，在大量VP-IR室旁和视上牛顿中也可见到ER-βmRNA的存在。在SON中，VP-IR神经元比OT-IR神经元有更强的杂交信号，这些结果支持了下丘脑室旁核和SON内OT和VP-IR神经元的功能直接受雌激素的调节，雌激素的基因组效应是由ER-β介导的观点。
The regulatory actions of estrogen on magnocellular oxytocin (OT) and vasopressin (VP) neurons of the paraventricular (PVN) and supraoptic (SON) nuclei are well documented. To date it is still debated whether the effect of estrogens is exerted directly or mediated by estrogen-sensitive interneurons. Previous immunocytochemical (ICC) and in situ hybridization (ISH) studies detected either law levels or absence of the classical estrogen receptor (ER-alpha) in the PVN and the SON of the rat. The present experiments using a combined ICC and ISH method were undertaken to examine the expression of the recently cloned beta form of ER (ER-beta) in OT- and VP-immunoreactive (IR) neuronal systems of the rat hypothalamus. The results demonstrate that the highest cellular levels of ER-beta messenger RNA (mRNA) in OT-IR neurons can be visualized in the caudal portion of the PVN and in an area ventro-medial to the central core of TTP-ITC cells. These neurons were previously shown to project caudally to the brain stem and the spinal cord to regulate autonomic functions. in addition, the whole rostro-caudal extent of the PVN and the SON contained OT-IR neurons that coexpressed variable levels of ER-beta mRNA. Similarly, the presence of ER-beta mRNA was seen in a large population of VP-IR paraventricular and supraoptic newtons. In the SON, somewhat stronger hybridization signal was detected in VP-IR neurons as compared with OT-IR neurons.Together, these findings provide strong support for the concept that the functions of OT- and VP-IR neurons in the PVN and the SON are regulated directly by estrogen and that the genomic effects of estrogens are mediated by ER-beta.