Sex differences and similarities in depressive-and anxiety-like behaviour in the Wistar-Kyoto rat
Sex differences and similarities in depressive-and anxiety-like behaviour in the Wistar-Kyoto rat
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DOI：
10.1016/j.physbeh.2016.08.031
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发表时间：
2016-12-01
影响因子：
2.9
通讯作者：
Kelly, John
中科院分区：
文献类型：
--
作者：
Burke, Nikita N.;Coppinger, Jonathan;Kelly, John
抑郁症是一种使人衰弱的精神疾病，与焦虑高度共病。抑郁症在女性中的发病率是男性的两倍，然而，女性在临床前研究中的代表性仍然不足。应激高反应性Wistar-Kyoto（WKY）大鼠在新的厌恶环境中表现出意识减退，并表现出抑郁和焦虑样行为，这些行为主要在雄性中表现出特征。目前的研究在一系列行为模式中描述了雄性和雌性大鼠的行为。成年雄性和雌性WKY大鼠在开放场地和强迫游泳试验（具有运动成分的试验）中进行了试验;还监测了大理石掩埋、新奇感诱导的摄食量减少和蔗糖偏好试验（具有最小运动成分的试验）以及24小时的笼内运动活动。将试验与Sprague-Dawley（SD）品系（一种常用的“对照”品系）进行比较。与雄性相比，SD雌性（而非WKY）表现出更高的笼内运动活性。在旷场中，与SD大鼠相比，两种性别的WKY大鼠表现出自发活动显著减少和焦虑样行为增加，表现为旷场厌恶内区的时间减少。在大理石掩埋测试中，WKY雌性动物（而非雄性动物）表现出掩埋增加的趋势，表明焦虑样/恐新行为。相比之下，WKY雄性大鼠（而非雌性大鼠）表现出新奇感诱导的食欲减退增强，表明与SD大鼠相比焦虑样行为增加。在强迫游泳试验中，与SD大鼠相比，两种性别的WKY大鼠花更多的时间不动，表明抑郁样行为。然而，与SD大鼠相比，WKY雄性大鼠（而非雌性大鼠）表现出快感缺失样行为。总之，WKY大鼠表现出抑郁和焦虑样行为，这些行为根据大鼠的性别和测试条件而复杂和微妙。本研究支持使用不同的测试组合，以充分验证雄性和雌性大鼠的抑郁/焦虑样行为。(C)2016 Elsevier Inc. All rights reserved.
Depression is a debilitating psychiatric disorder that is highly comorbid with anxiety. Depression is twice as prevalent in women as in men, however, females remain underrepresented in preclinical research. The stress hyper-responsive Wistar-Kyoto (WKY) rat displays hypolocotnotion in a novel aversive environment and depressive and anxiety-like behaviours, which have been mostly characterised in males. The current study characterised behaviour in male and female rats in a battery of behavioural paradigms. Adult male and female WKY rats were tested in the open field and forced swim tests (tests with a locomotor component); and the marble burying, novelty induced hypophagia and sucrose preference tests (tests with a minimal locomotor component) and 24 h home-cage locomotor activity was also monitored. The tests were compared against the Sprague-Dawley (SD) strain, a commonly used "control" strain.SD, but not WKY, females exhibited higher home-cage locomotor activity-compared to males. In the open field, WKY rats of both sexes exhibited a significant reduction in locomotor activity and increased anxiety-like behaviour as demonstrated by reduced time in the aversive inner zone of the open field, compared to SD counterparts. In the marble burying test, WKY females, but not males, exhibited a trend towards increased burying, indicative of anxiety-like/neophobic behaviour. In comparison, WKY males, but not females, exhibited enhanced novelty induced hypophagia, indicative of increased anxiety-like behaviour compared to SD rats. In the forced swim test, WKY rats of both sexes spent more time immobile compared with SD counterparts, indicating depressive-like behaviour. However, in comparison to SD rats, WKY males, but not females, exhibited anhedonic-like behaviour. In conclusion, WKY rats exhibit depressive-and anxiety-like behaviours that are complex and nuanced depending on the sex of the rat and testing conditions. This study supports the use of a varied test battery to fully characterise depression/anxiety-like behaviour in male and female rats. (C) 2016 Elsevier Inc. All rights reserved.