Quantification of vitamin C in the rat brain in vivo using short echo-time 1H MRS
Quantification of vitamin C in the rat brain in vivo using short echo-time 1H MRS
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DOI：
10.1002/mrm.20854
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发表时间：
2006-05-01
影响因子：
3.3
通讯作者：
Gruetter, R
中科院分区：
文献类型：
--
作者：
Terpstra, M;Tkác, I;Gruetter, R
超短回波时间STEAM和MEGA-PRESS编辑的光谱学都用于验证发育中大鼠大脑中维生素C（抗坏血酸盐）的无创定量，其中已报告了抗坏血酸盐浓度的变化。尽管与谷氨酰胺、谷氨酸、谷胱甘肽和大分子的共振有很强的重叠，但在9.4 T下，通过STEAM（TE = 2 ms）光谱的LC模型分析，可以可靠地定量抗坏血酸（Cramer-Rao下限<0.2 μ mol/g）。从STEAM光谱中定量的抗坏血酸浓度与从相同体积的目标测量的MEGA-PRESS编辑的光谱中完全分辨的抗坏血酸共振计算的浓度非常一致。使用蒸汽测量的抗坏血酸浓度随着出生后大鼠年龄的增加而降低，与使用高效液相色谱法（HPLC）在体外测量的已发表的脑抗坏血酸浓度一致。
Both ultrashort echo-time STEAM and MEGA-PRESS-edited spectroscopy were used to validate noninvasive quantification of vitamin C (ascorbate) in the developing rat brain, where changes in ascorbate concentration have been reported. Despite strong overlap with resonances from glutamine, glutamate, glutathione, and macromolecules, reliable quantification of ascorbate (Cramer-Rao lower bounds < 0.2 mu mol/g) by LC-Model analysis of STEAM (TE = 2 ms) spectra was possible at 9.4 T. Ascorbate concentrations quantified from the STEAM spectra were in very good agreement with concentrations calculated from fully resolved ascorbate resonances in MEGA-PRESS-edited spectra measured from identical volumes of interest. Ascorbate concentrations measured using STEAM decreased with increasing postnatal rat age, in agreement with published brain ascorbate concentrations measured in vitro using high-performance liquid chromatography (HPLC).