Enantioselective SERS sensing of pseudoephedrine in blood plasma biomatrix by hierarchical mesoporous Au films coated with a homochiral MOF
Enantioselective SERS sensing of pseudoephedrine in blood plasma biomatrix by hierarchical mesoporous Au films coated with a homochiral MOF
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DOI：
10.1016/j.bios.2021.113109
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发表时间：
2021-03-04
影响因子：
12.6
通讯作者：
Yamauchi, Yusuke
中科院分区：
文献类型：
--
作者：
Guselnikova, Olga;Lim, Hyunsoo;Yamauchi, Yusuke
在这里，我们提出了一类新的分级多孔杂化材料，作为一种创新的工具，通过手性表面增强拉曼光谱(SERS)对复杂生物样品中的对映体药物进行超灵敏和选择性的检测。将介孔等离子体金膜与微孔均手性金属有机骨架(HMOF)相结合，制备了多层多孔杂化薄膜。由于双重增强机制(介孔Au纳米结构的物理增强和HMOF的化学增强)、HMOF的化学识别以及对包含大生物分子(如血液成分)的生物样品的判别功能，所提出的分层多孔基质能够在未稀释的血浆中实现极低的检测值(10-12M)。我们展示了每个组分(介孔Au和微孔AlaZnCl(HMOF))对传感分析性能的影响。AlLaZnCl的生长导致SERS信号的增加(约17倍)，而使用介孔Au导致SERS信号的增加(最高可达40%)。在复杂的生物基质(血清或血浆)存在的情况下，混合分层多孔底物提供了对毛孔内分子运输的控制，并防止了血液蛋白质的渗透，在存在多组分生物基质的情况下，在检测极限和对映选择性方面引发了与现有血浆材料的竞争。
Here, we present a new family of hierarchical porous hybrid materials as an innovative tool for ultrasensitive and selective sensing of enantiomeric drugs in complex biosamples via chiral surface-enhanced Raman spectroscopy (SERS). Hierarchical porous hybrid films were prepared by the combination of mesoporous plasmonic Au films and microporous homochiral metal-organic frameworks (HMOFs). The proposed hierarchical porous substrates enable extremely low limit of detection values (10-12 M) for pseudoephedrine in undiluted blood plasma due to dual enhancement mechanisms (physical enhancement by the mesoporous Au nanostructures and chemical enhancement by HMOF), chemical recognition by HMOF, and a discriminant function for bio-samples containing large biomolecules, such as blood components. We demonstrate the effect of each component (mesoporous Au and microporous AlaZnCl (HMOF)) on the analytical performance for sensing. The growth of AlaZnCl leads to an increase in the SERS signal (by around 17 times), while the use of mesoporous Au leads to an increase in the signal (by up to 40%). In the presence of a complex biomatrix (blood serum or plasma), the hybrid hierarchical porous substrate provides control over the transport of the molecules inside the pores and prevents blood protein infiltration, provoking competition with existing plasmonic materials at the limit of detection and enantioselectivity in the presence of a multicomponent biomatrix.