HOMOLOGOUS BUT NOT HETEROLOGOUS CONTACT INCREASES THE INSULIN-SECRETION OF INDIVIDUAL PANCREATIC B-CELLS
HOMOLOGOUS BUT NOT HETEROLOGOUS CONTACT INCREASES THE INSULIN-SECRETION OF INDIVIDUAL PANCREATIC B-CELLS
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DOI：
10.1016/0014-4827(89)90365-0
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发表时间：
1989-09-01
影响因子：
3.7
通讯作者：
MEDA, P
中科院分区：
文献类型：
--
作者：
BOSCO, D;ORCI, L;MEDA, P
为了评估接触是否以及如何具体影响分化细胞的功能，我们研究了成人胰腺B细胞的分泌作为聚集到同源B细胞或其他异源内分泌胰岛细胞类型的函数，所有这些细胞都存在于混合细胞悬浮液中。使用免疫空斑测定胰岛素，我们已经定量的比例的单一和聚集的B细胞诱导形成的溶血性空斑（分泌细胞群体的大小的反映）和这些斑块的面积（反映的激素输出的个别细胞或聚集体）30分钟的刺激后，16.7 mM葡萄糖。通过考虑聚集体中B细胞的数量，我们从这些数据中计算了每个B细胞的胰岛素输出。我们在此表明，B细胞间的同源接触促进了分泌型B细胞的募集，并使它们的胰岛素分泌量比单个B细胞增加两倍。相比之下，异源B细胞与非B细胞接触在增强分泌B细胞的募集和促进其个体分泌方面无效。这些发现表明，高度分化的细胞功能，如胰岛素分泌，是由同源细胞与细胞接触特异性控制的。
To assess whether and how specifically contact influences the functioning of differentiated cells, we have studied the secretion of adult pancreactic B-cells as a function of aggregation to either homologous B-cells or other heterologous endocrine islet cell types, all present in a mixed cell suspension. Using an immunological plaque assay for insulin, we have quantitated the proportion of single and aggregated B-cells inducing the formation of a hemolytic plaque (a reflection of the size of the secreting cell population) and the area of these plaques (a reflection of the hormonal output of individual cells or aggregates) after a 30-min stimulation by 16.7 mM glucose. By taking into account the number of B-cells within the aggregates, we have calculated from these data the insulin output on a per B-cell basis. We show here that the homologous contact between companion B-cells promotes the recruitment of secreting B-cells and increases theier individual secretion of insulin twofold over that of single B-cells. By contrast, heterologous B- to non-B-cell contact was not effective in enhancing the recruitment of secreting B-cells and in promoting their individual secretion. These findins show that a highly differentiated cell function, such as insulin secretion, is controlled specifically by homologous cell to cell contacts.