Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1.
Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1.
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DOI：
10.1126/scisignal.2004143
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发表时间：
2013-09-03
影响因子：
7.3
通讯作者：
Dohlman HG
中科院分区：
文献类型：
--
作者：
Clement ST;Dixit G;Dohlman HG
细胞外信号，如营养物质和激素，提示细胞内通路产生适应性反应。通常，细胞必须协调它们对多种信号的反应，以产生适当的结果。我们发现葡萄糖感知途径的成分作用于酵母中异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）介导的信息素信号通路的成分。我们证明了G蛋白α亚基Gpa1在葡萄糖可用性降低的条件下被磷酸化，并且这种磷酸化事件有助于减少信息素依赖的有丝分裂原激活蛋白激酶、基因转录、细胞形态发生和交配效率的刺激。我们发现Elm1， Sak1和Tos3，这些激酶磷酸化Snf1（单磷酸腺苷活化蛋白激酶（AMPK）的酵母同源物），以响应有限的葡萄糖可用性，也磷酸化Gpa1，并导致交配反应减弱。Reg1是磷酸酶PP1的调控亚基，作用于Snf1，同样需要逆转Gpa1的磷酸化并维持交配反应。因此，调节Snf1的激酶和磷酸酶也调节Gpa1。更广泛地说，这些结果表明信息素信号和葡萄糖感应途径直接沟通以协调细胞行为。
Extracellular signals, such as nutrients and hormones, cue intracellular pathways to produce adaptive responses. Often, cells must coordinate their responses to multiple signals to produce an appropriate outcome. We showed that components of a glucose-sensing pathway acted on components of a heterotrimeric guanine nucleotide–binding protein (G protein)–mediated pheromone signaling pathway in the yeast Saccharomyces cerevisiae. We demonstrated that the G protein α subunit Gpa1 was phosphorylated in response to conditions of reduced glucose availability and that this phosphorylation event contributed to reduced pheromone-dependent stimulation of mitogen-activated protein kinases, gene transcription, cell morphogenesis, and mating efficiency. We found that Elm1, Sak1, and Tos3, the kinases that phosphorylate Snf1, the yeast homolog of adenosine monophosphate–activated protein kinase (AMPK), in response to limited glucose availability, also phosphorylated Gpa1 and contributed to the diminished mating response. Reg1, the regulatory subunit of the phosphatase PP1 that acts on Snf1, was likewise required to reverse the phosphorylation of Gpa1 and to maintain the mating response. Thus, the same kinases and phosphatase that regulate Snf1 also regulate Gpa1. More broadly, these results indicate that the pheromone signaling and glucose-sensing pathways communicate directly to coordinate cell behavior.
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影响因子：
16
作者：
Hao, Nan;Nayak, Sujata;Dohlman, Henrik G.
通讯作者：
Dohlman, Henrik G.
影响因子：
5.3
作者：
Hedbacker, K;Hong, SP;Carlson, M
通讯作者：
Carlson, M
DOI：
10.1073/pnas.95.11.6245
发表时间：
1998-05-26
影响因子：
11.1
作者：
Ludin, K;Jiang, R;Carlson, M
通讯作者：
Carlson, M
影响因子：
3.9
作者：
Nigavekar, SS;Tan, YSH;Cannon, JF
通讯作者：
Cannon, JF
影响因子：
2.9
作者：
Heenan, Erin J.;Vanhooke, Janeen L.;Dohlman, Henrik G.
通讯作者：
Dohlman, Henrik G.