Expression of GATA-binding proteins during embryonic development in Xenopus laevis.
Expression of GATA-binding proteins during embryonic development in Xenopus laevis.
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DOI：
10.1073/pnas.88.23.10642
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发表时间：
1991-12
影响因子：
11.1
通讯作者：
Leonard I. Zon;C. Mather;S. Burgess;M. E. Bolce;R. Harland;Stuart H. Orkin
中科院分区：
文献类型：
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作者：
Leonard I. Zon;C. Mather;S. Burgess;M. E. Bolce;R. Harland;Stuart H. Orkin
识别核心序列加塔的蛋白质是造血特异性基因转录的重要调节因子。我们已经鉴定了编码非洲爪蟾同源物的三个相关转录因子的cDNA，命名为加塔-1，-2和-3。比较序列分析表明，在所有脊椎动物GATA结合蛋白中锌指DNA结合结构域具有很强的保守性。加塔-2和加塔-3多肽在其整个序列中是同源的，而加塔-1序列仅在负责DNA结合的区域中是保守的。在非洲爪蟾中，编码GATA结合蛋白的RNA在幼虫和成体红系细胞中表达。加塔-1、-2和-3 RNA首先在早期原肠胚（Nieuwkoop发育阶段11）中检测到。这早于早期幼虫α T1珠蛋白RNA（阶段15）、β T1珠蛋白RNA（阶段26）或血岛形成（阶段30）的出现。加塔-1、-2和-3在早期发育中的表达可能是中胚层形成造血组织的早期定型信号。
Proteins that recognize the core sequence GATA are important regulators of hematopoietic-specific gene transcription. We have characterized cDNAs encoding the Xenopus laevis homologues of three related transcription factors, designated GATA-1, -2, and -3. Comparative sequence analysis reveals strong conservation of the zinc-finger DNA-binding domain among all vertebrate GATA-binding proteins. GATA-2 and GATA-3 polypeptides are homologous throughout their entire sequences, whereas GATA-1 sequence is conserved only in the region responsible for DNA binding. In Xenopus, RNAs encoding GATA-binding proteins are expressed in both larval and adult erythroid cells. GATA-1, -2, and -3 RNAs are first detectable in early gastrula (Nieuwkoop developmental stage 11). This is earlier than the appearance of the early larval alpha T1 globin RNA (stage 15), beta T1 globin RNA (stage 26), or blood island formation (stage 30). The expression of GATA-1, -2, and -3 in early development may signal an early commitment of mesoderm to form hematopoietic tissue.