PROTEIN MICROCRYSTAL DIFFRACTION AND THE EFFECTS OF RADIATION-DAMAGE WITH ULTRA-HIGH-FLUX SYNCHROTRON RADIATION
PROTEIN MICROCRYSTAL DIFFRACTION AND THE EFFECTS OF RADIATION-DAMAGE WITH ULTRA-HIGH-FLUX SYNCHROTRON RADIATION
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DOI：
10.1073/pnas.82.22.7604
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
PAPIZ, MZ
中科院分区：
文献类型：
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作者：
HEDMAN, B;HODGSON, KO;PAPIZ, MZ
利用摆动源的超高通量同步加速器x射线，获得了30倍尺寸的蛋白质微晶体的良好劳厄衍射数据。35个同学。10 .mu。M3，湿装在玻璃毛细血管中。在1013-1014光子/秒/平方毫米的通量水平下，辐射损伤仍然足够低，可以对微晶体的互反空间进行大规模调查，对正常大小的蛋白质晶体进行全面调查。因此，超高强度同步辐射源的开发是通过蛋白质晶体学以及在晶体尺寸通常是限制因素的其他情况下确定结构技术的重要改进。
By using ultra-high-flux synchrotron x-radiation from a wiggler source, good Laue diffraction data have been obtained from protein microcrystals of size 30 .times. 35 .times. 10 .mu.m3, mounted wet in glass capillaries. At the flux level of 1013-1014 photons per sec/mm2, the radiation damage is still low enough to allow a large survey of reciprocal space for a microcrystal and a complete survey for a normal-sized protein crystal. The development of sources for ultra-high-intensity synchrotron radiation is thus an important improvement in the technique for determination of structure through protein crystallography as well as in other cases where crystal size is often a limiting factor.