Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach.
Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach.
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DOI：
10.1016/j.envpol.2019.113333
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发表时间：
2019-12
影响因子：
8.9
通讯作者：
Lanlan Xu;Zhen Lu;Chenglong Ji;Ming Cong;Fei Li;X. Shan;Huifeng Wu
中科院分区：
文献类型：
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作者：
Lanlan Xu;Zhen Lu;Chenglong Ji;Ming Cong;Fei Li;X. Shan;Huifeng Wu
砷（As）是海洋环境中普遍存在的类金属元素，其潜在的毒性使其污染问题受到世界各国的关注。砷可引起动物脂质代谢紊乱、免疫系统功能紊乱、氧化应激和致癌等多种不良反应。无机砷在自然环境中有两种形态，即亚砷酸盐（As（III））和砷酸盐（As（V））。As（V）是天然沃茨中的主要形态。在本研究中，研究了幼年石鲈Sebastes schlegelii暴露于环境相关浓度的As（V）14 d后的代谢组学和蛋白质组学变化。基于iTRAQ的蛋白质组学结合非靶向NMR代谢组学分析表明，As（V）在幼鱼中诱导了明显的毒理学效应。具体而言，代谢产物，包括乳酸，丙氨酸，ATP，肌苷和磷酸胆碱在砷处理组中显着改变。蛋白质组学反应表明，As（V）不仅影响能量代谢、初级代谢和信号转导，而且影响细胞骨架结构。本研究表明，蛋白质组学和代谢组学相结合的方法可以揭示污染物对许氏平口鱼的毒理效应。
Arsenic (As) is a metalloid element that is ubiquitous in the marine environment and its contamination has received worldwide attention due to its potential toxicity. Arsenic can induce multiple adverse effects, such as lipid metabolism disorder, immune system dysfunction, oxidative stress and carcinogenesis, in animals. Inorganic arsenic includes two chemical forms, arsenite (As (III)) and arsenate (As (V)), in natural environment. As (V) is the dominant form in natural waters. In the present study, metabolomic and proteomic alterations were investigated in juvenile rockfishSebastes schlegeliiexposed to environmentally relevant concentrations of As (V) for 14 d. The analysis of iTRAQ-based proteomics combined with untargeted NMR-based metabolomics indicated apparent toxicological effects induced by As (V) in juvenile rockfish. In details, the metabolites, including lactate, alanine, ATP, inosine and phosphocholine were significantly altered in As-treated groups. Proteomic responses suggested that As (V) could not only affected energy and primary metabolisms and signal transduction, but also influenced cytoskeleton structure in juvenile rockfish. This work suggested that the combined proteomic and metabolomic approach could shed light on the toxicological effects of pollutants in rockfishS. schlegelii.